Exeter/30 July 2013

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== Miniprep, NanoDrop, SureClean of RBS + Cph8 (K592018)==
== Miniprep, NanoDrop, SureClean of RBS + Cph8 (K592018)==
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{| class="wikitable"
{| class="wikitable"
|-
|-
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! Culture !! Quickstart protocol (ng/ul) !! Sureclean (ng/ul)
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! Culture !! Quickstart protocol (ng/&micro;l) !! Sureclean (ng/&micro;l)
|-
|-
| RBS + Cph8 || 17.5 || 34.6
| RBS + Cph8 || 17.5 || 34.6
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| B0015 || 4.3 || 16.0
| B0015 || 4.3 || 16.0
|}
|}
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== Liquid cultures ==
== Liquid cultures ==
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* Positive controls of:
* Positive controls of:
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- RFP cut with E and S
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- RFP cut with <i>E</i> and <i>S</i>
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- RFP cut with X and P
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- RFP cut with <i>X</i> and <i>P</i>
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We got fresh purite water each time we needed it.
We got fresh purite water each time we needed it.
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We incubated at 37°C for 30 minutes and at 80°C for 20 minutes.
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We incubated at 37 &deg;C for 30 minutes and at 80 &deg;C for 20 minutes.
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Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook].
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Latest revision as of 21:56, 1 October 2013

Exeter iGEM 2013 · Paint by Coli

Miniprep, NanoDrop, SureClean of RBS + Cph8 (K592018)

NanoDrop + Sureclean data:

Culture Quickstart protocol (ng/µl) Sureclean (ng/µl)
RBS + Cph8 17.5 34.6
B0015 4.3 16.0

Liquid cultures

We made liquid cultures of:


Cph8 + RBS - K592018

B0015 - Terminator

K592022 - Cyan (w/RBS and promoter)

K864404 - Promoter, RBS and cyan

K592011 - Cyan pigment

S05058


Digest

We followed advice from a PhD student and did:

  • Positive controls of:

- RFP cut with E and S

- RFP cut with X and P


  • Negative control of:

- Just water


The buffer and BSA were vortexed before use to make sure they were completely defrosted.

We got fresh purite water each time we needed it.

We incubated at 37 °C for 30 minutes and at 80 °C for 20 minutes.

Take me back to the notebook.

Exeter iGEM 2013 · Paint by Coli
Retrieved from "http://2013.igem.org/Exeter/30_July_2013"