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Team:DTU-Denmark/Notebook/8 July 2013
From 2013.igem.org
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==Main purpose== | ==Main purpose== | ||
| - | Run gel with 1 AMO, 10 AMO, 20 AMO genes. | + | Run gel with 1 AMO, 10 AMO, 20 AMO genes from Nitrosomonas Europaea. |
Purification and extraction of the gel for 10 AMO. | Purification and extraction of the gel for 10 AMO. | ||
Run 2nd gel with all the sample of 10 AMO. | Run 2nd gel with all the sample of 10 AMO. | ||
| + | Run gel with HAO and CYC genes Nitrosomonas Europaea. | ||
| + | |||
==Who was in the lab== | ==Who was in the lab== | ||
Kristian, Ariadni, Gosia | Kristian, Ariadni, Gosia | ||
==Procedure== | ==Procedure== | ||
| - | Run 1st gel: | + | Run 1st gel (AMO): |
* 1ul of dye | * 1ul of dye | ||
* 5ul of sample | * 5ul of sample | ||
| Line 15: | Line 17: | ||
Purification of first gel for 10 AMO according to the protocol QIA gel extraction protocol. (given in the Kit) | Purification of first gel for 10 AMO according to the protocol QIA gel extraction protocol. (given in the Kit) | ||
| - | Run 2nd gel: | + | Run 2nd gel (AMO): |
* 9ul of dye | * 9ul of dye | ||
* around 20 ul of each sample | * around 20 ul of each sample | ||
| Line 23: | Line 25: | ||
Measure the concentration of the purified AMO using Nanodrop (pouring together both samples. | Measure the concentration of the purified AMO using Nanodrop (pouring together both samples. | ||
| + | |||
| + | Run 3rd gel (HAO,CYC): | ||
| + | * 1ul of dye | ||
| + | * 5ul of sample | ||
| + | * 5ul Kb ladder | ||
| + | |||
| + | Purification of DNA of both HAO and CYC. | ||
==Results== | ==Results== | ||
| - | Nanodrop measurement 30.4 ng/ul | + | Nanodrop measurement: |
| + | * 30.4 ng/ul (AMO) | ||
| + | * 14.4 ng/ul (HAO) | ||
| + | * 11.7 ng/ul (CYC) | ||
Revision as of 17:40, 8 July 2013
Contents |
208
Main purpose
Run gel with 1 AMO, 10 AMO, 20 AMO genes from Nitrosomonas Europaea. Purification and extraction of the gel for 10 AMO. Run 2nd gel with all the sample of 10 AMO. Run gel with HAO and CYC genes Nitrosomonas Europaea.
Who was in the lab
Kristian, Ariadni, Gosia
Procedure
Run 1st gel (AMO):
- 1ul of dye
- 5ul of sample
- 5ul Kb ladder
Purification of first gel for 10 AMO according to the protocol QIA gel extraction protocol. (given in the Kit)
Run 2nd gel (AMO):
- 9ul of dye
- around 20 ul of each sample
- 12 ul of ladder
Purification of second gel according to the same protocol.
Measure the concentration of the purified AMO using Nanodrop (pouring together both samples.
Run 3rd gel (HAO,CYC):
- 1ul of dye
- 5ul of sample
- 5ul Kb ladder
Purification of DNA of both HAO and CYC.
Results
Nanodrop measurement:
- 30.4 ng/ul (AMO)
- 14.4 ng/ul (HAO)
- 11.7 ng/ul (CYC)
