Template:Team:Uppsala/JS/notebook

Revision as of 19:06, 1 October 2013 (view source)

Revision as of 22:03, 1 October 2013 (view source)

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ds = '<div id="dairy-text"><h1>Sunday 2013-08-18</h1> Name of participants: Anders Edlund, Alexander, Pontus, Kristoffer L, Marcus H. <h2>Ongoing constructs:</h2> <h2>E. coli:</h2> 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work: </h2> ON: 134. pSB1C3-B0034-CBP 254. pSB1C3-gus 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.3 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.2 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry pSBLB4C15-red pSBLB4C15-red dam- Restreak: 68.1 pSB3K3-B0034-BFP 68.2 pSB3K3-B0034-BFP 68.4 pSB3K3-B0034-BFP 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 147.pSB1C3-B0034-Thioredoxin 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179 pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.pSB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.pSB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.pSB3K3-K206000-B0034-IspA 268.pSB3K3-K206001-B0034-IspA 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 300.pEL3S17-J23106-B0034-Thioredoxin PCR: 134. pSB1C3-B0034-CBP 254. pSB1C3-gus 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.3 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.2 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry Digest: 125. pSB1C3-ZCD-His 127. pSB1A3-B0034-UGTCs2-His 129.pSB1C3-Idi(mut) 184.pSB1C3-B0034-CrtZ Ligation: 4.pSB1A3-red 7.pSB3K3-red 36.pEL3S17-red 91.j23106 92.j23110 103.pSB1K3-j23101 125. pSB1C3-ZCD-His 127. pSB1A3-B0034-UGTCs2-His 129.pSB1C3-Idi(mut) 184.pSB1C3-B0034-CrtZ Plasmidprep: 184.pSB1C3-B0034-CrtZ Transformation: 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 302. idi (mut)-B0034-Ispa 303.pSB1A3-j23101-B0034-CrtZ 304.pSB1A3-j23106-B0034-CrtZ 305.pSB1A3-j23110-B0034-CrtZ O/N screening: 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 <h2>Results:</h2> Failed: 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 184.pSB1C3-B0034-CrtZ 202pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208.pSB1A3-B0034-Thioredoxin-ZCD-His 272 pSB1K3-B0034-Thioredoxin-ZCD-His 273 pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 274 pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His</div>'

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ds = '<div id="dairy-text"><h1>Monday 2013-08-19</h1> Name of participants: Anton Berglund, Viktor Blomkvist, Mikael Strandgren, Stephanie Herman, Kristoffer L, Marcus H, Karl H, Theodor L, Emil M, Victor S, Alexander, Pontus, Sabri <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 257. pSB1C3-pemp 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work: </h2> Screening 134.1 pSB1C3-CBP 254 pSB1C3-gus 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.3 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.2 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry Screening O/N: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 147.B0034-Thioredoxin 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 300.J23106-B0034-Thioredoxin 202.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 Digestion & screening: 254.1 pSB1C3-gus: XbaI & PstI 254.2 pSB1C3-gus: XbaI & PstI 255.1 pSBLB4C15-pemp: EcoRI & PstI 255.2 pSBLB4C15-pemp: EcoRI & PstI 257.1 pSB1C3-pemp: EcoRI & PstI 257.2 pSB1C3-pemp: EcoRI & PstI Frozen stock: 134.1 pSB1C3-CBP 275. pSBLB4C15-red (dam-) 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry 1 ml overnight culture: 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 139. pSB3K3-J23101-B0034-BFP Plasmid preparation 111.7(1) pSBLB4C15-red 111.7(2) pSBLB4C15-red 134.1 pSB1C3-CBP 275 pSBLB4C15-red (dam-) 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry Overnight culture: Lb11.2 plantarum 36E-no plasmid Phusion PCR: usp45 (synthesized signal peptide from Lactococcus lactis) Restreak 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ digest 36. pEL3S17-red Ligation 108.pEL3S17-J23106-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-His 141.pEL3S17-J23101-B0034-UGTCs2-His 142.pEL3S17-CP1-B0034-UGTCs2-His 143.pEL3S17-CP8-B0034-UGTCs2-His Subclone: 280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue 281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue 282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP 283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP Two step Assembly: 92. BBa_J61002-J23110, 149. pSB1C3-RBS-meffRed à 155. pSB1C3-J23110-RBS-meffRed 91. BBa_J61002-J23106, 149. pSB1C3-RBS-meffRed à 156. pSB1C3-J23106-RBS-meffRed 92. BBa_J61002-J23110, 150. pSB1C3-RBS-spisPink à 157. pSB1C3-J23110-RBS-spisPink 91. BBa_J61002-J23106, 150. pSB1C3-RBS-spisPink à 158.pSB1C3-J23106-RBS-spisPink 92. BBa_J61002-J23110, 148. pSB1C3-RBS-gfasPurple à 159.pSB1C3-J23110-RBS-gfasPurple 91. BBa_J61002-J23106, 148 pSB1C3-RBS-gfasPurple à 160.pSB1C3-J23106-RBS-gfasPurple <h2>Results:</h2> Screening 134.1 pSB1C3-CBP: showed the correct band! 254 pSB1C3-gus: showed a very weak band, not sure if correct! 288.1 pSB1C3-pem: showed the correct band! 288.2 pSB1C3-pem: showed the correct band! 288.3 pSB1C3-pem: did not show the correct band! 288.4 pSB1C3-pem: showed the correct band! 289.1 pSB1C3-mCherry: showed the correct band! 289.2 pSB1C3-mCherry: did not show the correct band! 289.3 pSB1C3-mCherry: showed the correct band! 289.4 pSB1C3-mCherry: showed the correct band. 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His: fail 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His: fail 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His: fail 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His: fail 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268: fail 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1: fail 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1: fail Digestion & screening: 254.1 pSB1C3-gus: XbaI & PstI, did not show the correct bands! 254.2 pSB1C3-gus: XbaI & PstI, did not show the correct bands! 255.1 pSBLB4C15-pemp: EcoRI & PstI, showed the correct bands! 255.2 pSBLB4C15-pemp: EcoRI & PstI, showed the correct bands! 257.1 pSB1C3-pemp: EcoRI & PstI, showed the correct bands! 257.2 pSB1C3-pemp: EcoRI & PstI, showed the correct bands! Plasmid preparation 111.7(1) pSBLB4C15-red: 81.7 ng/µl 111.7(2) pSBLB4C15-red: 93.9 ng/µl 134.1 pSB1C3-CBP: 163.8 ng/µl 275 pSBLB4C15-red (dam-): 92.4 ng/µl 288.1 pSB1C3-pem: 211.2 ng/µl 288.2 pSB1C3-pem: 193.2 ng/µl 288.4 pSB1C3-pem: 193.9 ng/µl 289.1 pSB1C3-mCherry: 230.4 ng/µl 289.3 pSB1C3-mCherry: 229.7 ng/µl 289.4 pSB1C3-mCherry: 239.7 ng/µl</div>'

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}

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else if(id == 'd2013820')

+

{

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ds = '<div id="dairy-text"><h1>Tuesday 2013-08-20</h1> Name of participants: Anton Berglund, Stephanie Herman, Alona Nyberg, Alexander, Pontus, Sabri, Malin, Magnus, Marcus H, Karl H, Theodor L, Emil M, Victor S <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 257. pSB1C3-pemp 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 208. pSB1A3-B0034-Thioredoxin-ZCD-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His (201. E.Coli. Nissle - CrtY-CrtI) 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 147.pSB1C3-B0034-Thioredoxin 184.pSB1C3-B0034-CrtZ 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work: </h2> Characterisation of the CP promoters with facs: 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP Digestion: USP. with E, P 191. pSB1A2-fusionGFP with X, P 280. pSB1A3-B0034-aeBlue with X, P 147.B0034-Thioredoxin (E, S) 147.B0034-Thioredoxin (X,P) 27.pEL3K16-red (E,P) 125.pSB1C3-ZCD-His (X,P) Ligation: 208. pSB1A3-B0034-Thioredoxin-ZCD-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin Transformation: 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ Screen/ ON: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1A3-j23101-B0034-CrtZ 304.pSB1A3-j23106-B0034-CrtZ Plasmid prep. / Seq. prep. 147.pSB1C3-B0034-Thioredoxin 184.pSB1C3-B0034-CrtZ Gel: 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 207.CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 Phusion PCR: ->219.pSB1C3-Miraculin Making competent Lactobacillus reuteri Lb12.1 reuteri DSM 20016 plasmid free: eight Lb12.2 reuteri DSM 20016 plasmid free: eight Electroporation Lb3.2 reuteri DSM 20016 pVS2: two 111.7(1) pSBLB4C15-red: one 111.7(2) pSBLB4C15-red: two 255.1 pSBLB4C15-pemp: one 255.2 pSBLB4C15-pemp: one 275 pSBLB4C15-red (dam-): three <h2>Results:</h2> Electroporation Lb3.2 reuteri DSM 20016 pVS2: one of the two survived 111.7(1) pSBLB4C15-red: survived 111.7(2) pSBLB4C15-red: one of the two survived 255.1 pSBLB4C15-pemp: survived 255.2 pSBLB4C15-pemp: survived 275 pSBLB4C15-red (dam-): two of the three survived</div>'

+

}

+

else if(id == 'd2013821')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-08-21</h1> Name of participants: Anton Berglund, Mikael Strandgren, Viktor Blomkvist, Alexander, Pontus, Sabri, Malin, Magnus, christoffer, Marcus H, Karl H, Theodor L, Emil M, Victor S, Hampus.E, Lovisa P <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 257. pSB1C3-pemp 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 204(1-4). pEL3S17-J23110-B0034-His-STS E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work: </h2> Digestion: usp45: XbaI & AgeI 191.1 pSB1A2-fusionGFP: XbaI, PstI 191.1 pSB1A2-fusionGFP: NgoMIV & PstI 275. pSBLB4C15-red (dam-): EcoRI & PstI 289.1 pSB1C3-mCherry: NgoMIV & PstI 289.1 pSB1C3-mCherry: XbaI, PstI 297.1 pSBLB4B15-red (dam-): EcoRI & PstI Ligation: pSBLB4B15-CP11-fusionGFP (297.1+63.2+191.1) pSBLB4B15-CP30-fusionGFP (297.1+65.1+191.1) pSBLB4B15-CP11-usp45-fusionGFP (297.1+63.2+usp45+191.1) pSBLB4B15-CP30-usp45-fusionGFP (297.1+65.2+usp45+191.1) pSBLB4B15-CP11-usp45-mCherry (297.1+63.2+usp45+289.1) pSBLB4B15-CP30-usp45-mCherry (297.1+65.2+usp45+289.1) pSB1C3-usp45 (1+usp45) pSB1C3-usp45 (6+usp45) pSBLb4C15-CP1-B0034-BFP pSBLb4C15-CP8-B0034-BFP pSBLb4C15-CP11-B0034-BFP pSBLb4C15-CP29-B0034-BFP pSBLb4C15-CP30-B0034-BFP pSBLb4C15-CP41-B0034-BFP pSBLb4C15-CP44-B0034-BFP pSBLb4C15-CP11-aeblue pSBLb4C15-CP30-aeblue pSBLb4B15-CP11-mcherry pSBLb4B15-CP30-mcherry pSBLb4B15-CP11-tspurple pSBLb4B15-CP30-tspurple pSBLb4B15-CP11-amilCP pSBLb4B15-CP30-amilCP pSBLb4B15-CP11-amilGFP pSBLb4B15-CP30-amilGFP 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 265.pSB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.pSB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin 303.pSB1A3-j23101-B0034-CrtZ 304.pSB1A3-j23106-B0034-CrtZ Transformation: 108.pEL3S17-J23106-B0034-IspA 141.pRL3S17-J23101-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 208.pSB1A3-B0034-Thioredoxin-ZCD-His 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 272.pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303.pSB1A3-j23101-B0034-CrtZ 304.pSB1A3-j23106-B0034-CrtZ 204(1-4) Re-streak: 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink Screen/ ON: 219.pSB1C3-Miraculin 304.pSB1A3-J23106-B0034-CrtZ 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 280. pSB1A3-B0034-aeBlue 281. pSB1K3-J23110-B0034-aeBlue 282. pSB3K3-J23110-B0034-amilCP 283. pSB3K3-J23110-B0034-asCP Plasmid prep. 110.pEL3S17-K206001-B0034-IspA 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His Mutagenesis PCR: 336. pSB1C3-spisPink 337. pSB1C3-scOrange 338. pSB1C3-meffBlue 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 257. pSB1C3-pemp 288. pSB1C3-pem <h2>Other experiments:</h2> Phosphorylation of primers (20µl of each): Pem-mutE_F Pem-mutE_R Pem-mutS_F Pem-mutS_R Cleaning of electroporation cuvettes</div>'

+

}

+

else if(id == 'd2013822')

+

{

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ds = '<div id="dairy-text"><h1>Thursday 2013-08-22</h1> Name of participants: Anders Edlund, Anton Berglund, Mikael Strandgren, Viktor Blomkvist, Stephanie Herman, Alona Nyberg, Alexander, Pontus, Sabri, Malin, Magnus <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 257. pSB1C3-pemp 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 208. pSB1A3-B0034-Thioredoxin-ZCD-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His (201. E.Coli. Nissle - CrtY-CrtI) 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 147.pSB1C3-B0034-Thioredoxin 184.pSB1C3-B0034-CrtZ 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 115. pSB3K5-J23101-B0034-His-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL (from 15) E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work:</h2> Competent cell preparation: Lb11.1 plantarum 36E no plasmid Lb12.1 reuteri DSM 20016 no plasmid Lb12.2 reuteri DSM 20016 no plasmid Digest: 147.B0034-Thioredoxin (E, S) 147.B0034-Thioredoxin (X,P) 27.pEL3K16-red (E,P) 125.pSB1C3-ZCD-His (X,P) Ligation: 208. pSB1A3-B0034-Thioredoxin-ZCD-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin Transformation: 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ Lb31. plantarum 36E pVS2 Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple Lb35. reuteri DSM 20016 pVS2 Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP <strike>3.2. pVS2 (died in electroporation) 310. pSBLB4B15-CP11-fusionGFP (died in electroporation) 318. pSBLB4B15-CP11-mCherry (died in electroporation) 320. pSBLB4B15-CP11-amilGFP (died in electroporation) 313. pSBLB4B15-CP30-usp45-fusionGFP (died in electroporation) 317. pSBLB4B15-CP30-tsPurple (died in electroporation) 331. pSBLB4C15-CP41-BFP (died in electroporation) 332. pSBLB4C15-CP44-BFP (died in electroporation)</strike> + other E. coli transformations Mutagenesis PCR: 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R Screen/ ON: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1A3-j23101-B0034-CrtZ 304.pSB1A3-j23106-B0034-CrtZ Plasmid prep. / Seq. prep. 147.pSB1C3-B0034-Thioredoxin 184.pSB1C3-B0034-CrtZ Gel: 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 207.CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 Phusion PCR: ->219.pSB1C3-Miraculin <h2>Results:</h2> Mutagenesis PCR: 257. pSB1C3-pemp: Problem with PCR-machine, paused at 98*C for 25 min. → re-do. 288. pSB1C3-pem: --”-- Transformation: 11.1 Lb. plantarum 36E: 3.2 pVS2: survived electroporation 3.2 pVS2: died during electroporation 310. pSBLB4B15-CP11-fusionGFP: died during electroporation 311. pSBLB4B15-CP30-fusionGFP: survived electroporation 312. pSBLB4B15-CP11-usp45-fusionGFP: survived electroporation 313. pSBLB4B15-CP30-usp45-fusionGFP: died during electroporation 316. pSBLB4B15-CP11-tsPurple: survived electroporation 317. pSBLB4B15-CP30-tsPurple: died during electroporation 12.2 Lb. reuteri DSM 20016: 3.2 pVS2: survived electroporation 318. pSBLB4B15-CP11-mCherry: died during electroporation 319. pSBLB4B15-CP30-mCherry: survived electroporation 320. pSBLB4B15-CP11-amilGFP: died during electroporation 321. pSBLB4B15-CP30-amilGFP: survived electroporation 322. pSBLB4B15-CP11-amilCP: survived electroporation 323. pSBLB4B15-CP30-amilCP: survived electroporation 324. pSBLB4C15-CP11-aeBlue: survived electroporation 12.1 Lb. reuteri DSM 20016: 325. pSBLB4C15-CP30-aeBlue: survived electroporation 326. pSBLB4C15-CP1-BFP: survived electroporation 327. pSBLB4C15-CP8-BFP: survived electroporation 328. pSBLB4C15-CP11-BFP: survived electroporation 329. pSBLB4C15-CP29-BFP: survived electroporation 330. pSBLB4C15-CP30-BFP: survived electroporation 331. pSBLB4C15-CP41-BFP: died during electroporation 332. pSBLB4C15-CP44-BFP: died during electroporation <h2>Characterization:</h2> Western blot analysis (SDS-page) on constructs: 115. pSB3K5-J23101-B0034-His-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL (from 15) (neg.contr) <h2>Other experiments:</h2> Phosphorylation of primers (60 µl of each): Pem-mutE_F Pem-mutE_R Pem-mutS_F Pem-mutS_R</div>'

+

}

+

else if(id == 'd2013823')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-08-23</h1> Name of participants: Anton Berglund, Stephanie Herman, Viktor Berglund, Mikael Strandgren, Anders Edlund, Alexander, Pontus, Sabri, Malin, Magnus, Marcus H, Karl H, Hampus.E, Lovisa P, Kristoffer L, Emil M <h2>Ongoing constructs:</h2> E. coli (D5α): 111.7 pSBLb4C15-red 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS <h2>Todays work:</h2> Gel electrophoresis: 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R 111.7 pSBLb4C15-red (cut with E and P) (to get reference/examine cutsites) Mutagenesis PCR: 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R Touchdown with Tm1+2 and Tm1+4. Transformation: 102.pSB1C3-CrtE 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 208. pSB1A3-B0034-Thioredoxin-ZCD-His 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 223.pSB3K3-CP8-B0034-UGTCs2-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 300. pEL3S17-J23106-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin Re-streak: 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow Screening O/N: 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 222.pSB3K3-CP1-B0034-UGTCs2-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 301. pEL3S17-J23110-B0034-Thioredoxin O/N: 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink Subclone: 280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue 281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue 282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP 283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP Glycerol stock Plasmidprep/sekvensprep: 108.pEL3S17-J23106-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 184.pSB1C3-B0034-CrtZ 299.pEL3S17-J23101-B0034-Thioredoxin <h2>Results:</h2> Gel electrophoresis: 257. pSB1C3-pemp with Pem-mutE_F & Pem-mutE_R: Unspecific binding, nc ok!* 288. pSB1C3-pem with Pem-mutE_F & Pem-mutE_R: Unspecific binding, nc ok!* 111.7 pSBLb4C15-red (cut with E and P): No band *→ redo mutagenesis PCR with higher temperatures</div>'

+

}

+

else if(id == 'd2013824')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-08-24<h1> Name of participants: Mikael Strandgren, Anders Berglund, Alexander, Pontus, Sabri, Magnus, Marcus H, Karl H, Hampus.E, Lovisa P, Kristoffer L, Emil M <h2>Ongoing constructs:</h2> Escherichia coli 257. pSB1C3-pemp 288. pSB1C3-pem 136. pSB1C3-B0034-USP pSB1C3-B0034-usp45 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS Lactobacillus reuteri: Lb35. reuteri DSM 20016 pVS2 Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP Lactobacillus plantarum: Lb31. plantarum 36E pVS2 Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple <h2>Todays work:</h2> Mutagenesis 338.pSB1C3-meffBlue Digest: 200.pSB1C3-B0034-asCP E,X 148.pSB1C3-RBS-gfasPurple E,X 20.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 E,X 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R (EcoRI) 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R (EcoRI) Ligation: 200.pSB1C3-B0034-asCP+91.BBa_J61002 (amp)-J23106→ → 343.J23106-B0034-asCP 148.pSB1C3-RBS-gfasPurple+91.BBa_J61002 (amp)-J23106→ → 160.j23106-RBS-gfasPurple 148.pSB1C3-RBS-gfasPurple+92.BBa_J61002 (amp)-J23110→ → 159j23110-RBS-gfasPurple 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1+6.pSB1K3-red→ →215.pSB1K3-pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 184.pSB1C3-B0034-CrtZ+6.pSB1K3-Red+103.pSB1K3-J23101→ → 303.pSB1K3-J23101-B0034-CrtZ 127.pSB1A3-B0034-UGTCs2-His+7.pSB3K3-Red+CP8→ → 223.pSB3K3-CP8-B0034-UGTCs2-His Transformation: 223.pSB3K3-CP8-B0034-UGTCs2-His 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 208.pSB1A3-B0034-Thioredoxin-ZCD-His 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 343.pSB1C3-J23106-B0034-asCP 160.pSB1C3-j23106-RBS-gfasPurple 159.pSB1C3-j23110-RBS-gfasPurple 303.pSB1K3-j23101-B0034-CrtZ 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 336.pSB1C3.spisPink 337.pSB1C3.scOrange 284.pSB1C3.B0034-aeBlue 285.pSB1C3.J23110-B0034-aeBlue 286.pSB1C3.J23110-B0034-amilCP 287.pSB1C3-J23110-B0034-asCP 336.pSB1C3-spisPink 337.pSB1C3-scOrange 284. pSB1C3-B0034-aeBlue 285. pSB1C3-J23110-B0034-aeBlue 286. pSB1C3-J23110-B0034-amilCP 287. pSB1C3-J23110-B0034-asCP O/N screening: 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow Lb31. plantarum 36E pVS2 Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple Lb35. reuteri DSM 20016 pVS2 Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP 136. 312 313 315 321 325 327 332 317 O/N: 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 143. pEL3S17-CP8-B0034-UGTCs2-6xHis 179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 265. J23110-B0034-Exp-Linker-Miraculin-His 301. pEL3S17-J23110-B0034-Thioredoxin Plasmid prepp & sequence prepp 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink Gel electrophoresis: Lb31. plantarum 36E pVS2 Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple Lb35. reuteri DSM 20016 pVS2 Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R + E. coli transformations PCR-purification: 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R DpnI: 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R <h2>Results:</h2> Transformations: All transformations spread on Bleomycin/zeocin have grown! If they are the right lactobacilli with the right plasmid remains to be seen, there seem to be a lot of contamination in the form of “clouds” covering all plates. Yet still there are larger colonies that very well could be what we want. Maybe the antibiotics are not efficient enough, we will try to spread normal plasmid free reuteri from frozen stock on the plates to investigate… We’ll also try a colony screening today with VF2 and VR. Nothing grew on chloramphenicol plates, not even the positive control pVS2, though it grew on erythromycin both when transformed into reuteri and plantarum. Gel electrophoresis: All Pem/Pemp mutagenesises showed bands at the right lengths (~2700 bp), although they were a bit smudgy - weakly stretching backward toward the wells. The digests of Pem simply showed one band, a weaker counterpart of the uncut PCR-product. We suspect the digest didn’t go as supposed, maybe due to the PCR buffer. The lactobacillus colony screenings showed up empty, perhaps the cells didn’t lyse? Or didn’t contain plasmids with VF and VR… We will continue to investigate the resistance problems, and await the sequencing results of the insertsite. The screenings of e. coli transformations done thursday also gave us an empty gel, without even ladders. Possibly the gel had to shallow wells.</div>'

+

}

+

else if(id == 'd2013825')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-08-25</h1> Name of participants: Alexander, Pontus, Sabri, Malin, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs:</h2> 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS <h2>Todays work:</h2> Mutagenesis 338.pSB1C3-meffBlue Plasmidprepp: 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 265.pSB3K3.J23110-B0034-Exp-Linker-Miraculin-His 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 143. pEL3S17-CP8-B0034-UGTCs2-6xHis 179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 265. J23110-B0034-Exp-Linker-Miraculin-His 301. pEL3S17-J23110-B0034-Thioredoxin Digest: 147.pSB1C3-B0034-Thioredoxin Ligate: 147.pSB1C3-B0034-Thioredoxin+CP1→ → 346.pSB1C3-CP1-B0034-Thioredoxin 147.pSB1C3-B0034-Thioredoxin+CP8→ →347.pSB1C3-CP8-B0034-Thioredoxin 147.pSB1C3-B0034-Thioredoxin+CP41→ →348.pSB1C3-CP41-B0034-Thioredoxin Transformation: 1.pSB1C3-Red (competens test) 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 208.pSB1A3-B0034-Thioredoxin-ZCD-His 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 223.pSB3K3-CP8-B0034-UGTCs2-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 285.pSB1C3.J23110-B0034-aeBlue 286.pSB1C3.J23110-B0034-amilCP 287.pSB1C3-J23110-B0034-asCP303.pSB1K3-j23101-B0034-CrtZ 336.pSB1C3-spisPink 337pSB1C3-scOrange 346.pSB1C3-CP1-B0034-Thioredoxin 347.pSB1C3-CP8-B0034-Thioredoxin 348.pSB1C3-CP41-B0034-Thioredoxin 285. pSB1C3-J23110-B0034-aeBlue 286. pSB1C3-J23110-B0034-amilCP 287. pSB1C3-J23110-B0034-asCP 336.pSB1C3-spisPink 337.pSB1C3-scOrange 159. pSB1C3-J23110-RBS-gfasPurple 160. pSB1C3-J23110-RBS-gfasPurple Re-streak: 284. pSB1C3-B0034-aeBlue Sequenceprep: 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) </div>'

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else if(id == 'd2013919')

{

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 	ds = '<div id="dairy-text"><h1>Sunday 2013-08-18</h1><br><b>Name of participants: </b>Anders Edlund, Alexander, Pontus, Kristoffer L, Marcus H.<br><br><h2>Ongoing constructs:</h2><br><h2>E. coli:</h2><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work: </h2><br><b>ON:</b><br>134. pSB1C3-B0034-CBP<br>254. pSB1C3-gus<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.3 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.2 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br>pSBLB4C15-red<br>pSBLB4C15-red dam-<br><br><b>Restreak:</b><br>68.1 pSB3K3-B0034-BFP<br>68.2 pSB3K3-B0034-BFP<br>68.4 pSB3K3-B0034-BFP<br>109.pEL3S17-K206000-B0034-IspA <br>110.pEL3S17-K206001-B0034-IspA <br>147.pSB1C3-B0034-Thioredoxin<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His <br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His <br>179 pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His <br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His <br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His <br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His <br>265.pSB3K3-J23110-B0034-Exp-Linker-Miraculin-His <br>266.pSB3K3-CP1-B0034-Exp-Linker-Miraculin-His <br>267.pSB3K3-K206000-B0034-IspA <br>268.pSB3K3-K206001-B0034-IspA <br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His <br>300.pEL3S17-J23106-B0034-Thioredoxin<br><br><b>PCR:</b> <br>134. pSB1C3-B0034-CBP<br>254. pSB1C3-gus<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.3 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.2 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br><br><b>Digest:</b><br>125. pSB1C3-ZCD-His<br>127. pSB1A3-B0034-UGTCs2-His<br>129.pSB1C3-Idi(mut) <br>184.pSB1C3-B0034-CrtZ<br><br><b>Ligation:</b><br>4.pSB1A3-red<br>7.pSB3K3-red<br>36.pEL3S17-red<br>91.j23106<br>92.j23110<br>103.pSB1K3-j23101<br>125. pSB1C3-ZCD-His<br>127. pSB1A3-B0034-UGTCs2-His<br>129.pSB1C3-Idi(mut) <br>184.pSB1C3-B0034-CrtZ<br><br><b>Plasmidprep:</b><br>184.pSB1C3-B0034-CrtZ<br><br><b>Transformation:</b><br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>302. idi (mut)-B0034-Ispa<br>303.pSB1A3-j23101-B0034-CrtZ<br>304.pSB1A3-j23106-B0034-CrtZ<br>305.pSB1A3-j23110-B0034-CrtZ<br><br><b>O/N screening:</b><br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><br><h2>Results:</h2><br><b>Failed:</b><br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His <br>184.pSB1C3-B0034-CrtZ<br>202pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His <br>272 pSB1K3-B0034-Thioredoxin-ZCD-His <br>273 pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His <br>274 pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His</div>'
   }
+      else if(id == 'd2013819')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-08-19</h1><br><b>Name of participants: </b>Anton Berglund, Viktor Blomkvist, Mikael Strandgren, Stephanie Herman, Kristoffer L, Marcus H, Karl H, Theodor L, Emil M, Victor S, Alexander, Pontus, Sabri<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>257. pSB1C3-pemp<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work: </h2><br><b>Screening</b><br>134.1 pSB1C3-CBP<br>254 pSB1C3-gus<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.3 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.2 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br><br><b>Screening O/N:</b><br>109.pEL3S17-K206000-B0034-IspA <br>110.pEL3S17-K206001-B0034-IspA <br>147.B0034-Thioredoxin<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His <br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His <br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His <br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His <br>267.psB3K3-K206000-B0034-IspA <br>268.psB3K3-K206001-B0034-IspA <br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His <br>300.J23106-B0034-Thioredoxin<br>202.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><br><b>Digestion & screening:</b><br>254.1 pSB1C3-gus: XbaI & PstI<br>254.2 pSB1C3-gus: XbaI & PstI<br>255.1 pSBLB4C15-pemp: EcoRI & PstI<br>255.2 pSBLB4C15-pemp: EcoRI & PstI<br>257.1 pSB1C3-pemp: EcoRI & PstI<br>257.2 pSB1C3-pemp: EcoRI & PstI<br><br><b>Frozen stock:</b><br>134.1 pSB1C3-CBP<br>275. pSBLB4C15-red (dam-)<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br><br><b>1 ml overnight culture:</b><br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>139. pSB3K3-J23101-B0034-BFP<br><br><b>Plasmid preparation</b><br>111.7(1) pSBLB4C15-red<br>111.7(2) pSBLB4C15-red<br>134.1 pSB1C3-CBP<br>275 pSBLB4C15-red (dam-)<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br><br><b>Overnight culture:</b><br>Lb11.2 plantarum 36E-no plasmid<br><br><b>Phusion PCR:</b><br>usp45 (synthesized signal peptide from Lactococcus lactis)<br><br><b>Restreak</b><br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br><br><b>digest</b><br>36. pEL3S17-red<br><br><b>Ligation</b><br>108.pEL3S17-J23106-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-His<br>141.pEL3S17-J23101-B0034-UGTCs2-His<br>142.pEL3S17-CP1-B0034-UGTCs2-His<br>143.pEL3S17-CP8-B0034-UGTCs2-His<br><br><b>Subclone:</b><br>280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue<br>281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue<br>282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP<br>283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP<br><br><b>Two step Assembly:</b><br>92. BBa_J61002-J23110,  149. pSB1C3-RBS-meffRed à 155. pSB1C3-J23110-RBS-meffRed<br>91. BBa_J61002-J23106,  149. pSB1C3-RBS-meffRed à 156. pSB1C3-J23106-RBS-meffRed<br>92. BBa_J61002-J23110, 150. pSB1C3-RBS-spisPink à 157. pSB1C3-J23110-RBS-spisPink<br>91. BBa_J61002-J23106, 150. pSB1C3-RBS-spisPink à 158.pSB1C3-J23106-RBS-spisPink<br>92. BBa_J61002-J23110, 148. pSB1C3-RBS-gfasPurple à 159.pSB1C3-J23110-RBS-gfasPurple<br>91. BBa_J61002-J23106, 148 pSB1C3-RBS-gfasPurple à 160.pSB1C3-J23106-RBS-gfasPurple<br><br><h2>Results:</h2><br><b>Screening</b><br>134.1 pSB1C3-CBP: showed the correct band!<br>254 pSB1C3-gus: showed a very weak band, not sure if correct!<br>288.1 pSB1C3-pem: showed the correct band!<br>288.2 pSB1C3-pem: showed the correct band!<br>288.3 pSB1C3-pem: did not show the correct band!<br>288.4 pSB1C3-pem: showed the correct band!<br>289.1 pSB1C3-mCherry: showed the correct band!<br>289.2 pSB1C3-mCherry: did not show the correct band!<br>289.3 pSB1C3-mCherry: showed the correct band!<br>289.4 pSB1C3-mCherry: showed the correct band.<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His: fail<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His: fail<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His: fail<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His: fail<br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268: fail<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1: fail<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1: fail<br><br><b>Digestion & screening:</b><br>254.1 pSB1C3-gus: XbaI & PstI, did not show the correct bands!<br>254.2 pSB1C3-gus: XbaI & PstI, did not show the correct bands!<br>255.1 pSBLB4C15-pemp: EcoRI & PstI, showed the correct bands!<br>255.2 pSBLB4C15-pemp: EcoRI & PstI, showed the correct bands!<br>257.1 pSB1C3-pemp: EcoRI & PstI, showed the correct bands!<br>257.2 pSB1C3-pemp: EcoRI & PstI, showed the correct bands!<br><br><b>Plasmid preparation</b><br>111.7(1) pSBLB4C15-red: 81.7 ng/µl<br>111.7(2) pSBLB4C15-red: 93.9 ng/µl<br>134.1 pSB1C3-CBP: 163.8 ng/µl<br>275 pSBLB4C15-red (dam-): 92.4 ng/µl<br>288.1 pSB1C3-pem: 211.2 ng/µl<br>288.2 pSB1C3-pem: 193.2 ng/µl<br>288.4 pSB1C3-pem: 193.9 ng/µl<br>289.1 pSB1C3-mCherry: 230.4 ng/µl<br>289.3 pSB1C3-mCherry: 229.7 ng/µl<br>289.4 pSB1C3-mCherry: 239.7 ng/µl</div>'
+ }
+      else if(id == 'd2013820')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-08-20</h1><br><b>Name of participants: </b>Anton Berglund, Stephanie Herman, Alona Nyberg, Alexander, Pontus, Sabri, Malin, Magnus, Marcus H, Karl H, Theodor L, Emil M, Victor S<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>257. pSB1C3-pemp<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>147.pSB1C3-B0034-Thioredoxin<br>184.pSB1C3-B0034-CrtZ<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work: </h2><br><b>Characterisation of the CP promoters with facs:</b><br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br><br><b>Digestion:</b><br>USP. with E, P<br>191. pSB1A2-fusionGFP with X, P<br>280. pSB1A3-B0034-aeBlue with X, P <br>147.B0034-Thioredoxin (E, S)<br>147.B0034-Thioredoxin (X,P)   <br>27.pEL3K16-red (E,P)<br>125.pSB1C3-ZCD-His (X,P)<br><br><b>Ligation:</b><br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><b>Transformation:</b><br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br><br><b>Screen/ ON:</b><br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1A3-j23101-B0034-CrtZ<br>304.pSB1A3-j23106-B0034-CrtZ<br><br><b>Plasmid prep. / Seq. prep.</b><br>147.pSB1C3-B0034-Thioredoxin<br>184.pSB1C3-B0034-CrtZ<br><br><b>Gel:</b><br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>207.CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><br><b>Phusion PCR:</b><br>->219.pSB1C3-Miraculin<br><br><b>Making competent Lactobacillus reuteri</b><br>Lb12.1 reuteri DSM 20016 plasmid free: eight<br>Lb12.2 reuteri DSM 20016 plasmid free: eight<br><br><b>Electroporation</b><br>Lb3.2 reuteri DSM 20016 pVS2: two<br>111.7(1) pSBLB4C15-red: one<br>111.7(2) pSBLB4C15-red: two<br>255.1 pSBLB4C15-pemp: one<br>255.2 pSBLB4C15-pemp: one<br>275 pSBLB4C15-red (dam-): three<br><br><h2>Results:</h2><br><b>Electroporation</b><br>Lb3.2 reuteri DSM 20016 pVS2: one of the two survived<br>111.7(1) pSBLB4C15-red: survived<br>111.7(2) pSBLB4C15-red: one of the two survived<br>255.1 pSBLB4C15-pemp: survived<br>255.2 pSBLB4C15-pemp: survived <br>275 pSBLB4C15-red (dam-): two of the three survived</div>'
+ }
+      else if(id == 'd2013821')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-08-21</h1><br><b>Name of participants: </b>Anton Berglund, Mikael Strandgren, Viktor Blomkvist, Alexander, Pontus, Sabri, Malin, Magnus, christoffer, Marcus H, Karl H, Theodor L, Emil M, Victor S, Hampus.E, Lovisa P<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>257. pSB1C3-pemp<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>204(1-4). pEL3S17-J23110-B0034-His-STS<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work: </h2><br><b>Digestion:</b><br>usp45: XbaI & AgeI<br>191.1 pSB1A2-fusionGFP: XbaI, PstI<br>191.1 pSB1A2-fusionGFP: NgoMIV & PstI<br>275. pSBLB4C15-red (dam-): EcoRI & PstI<br>289.1 pSB1C3-mCherry: NgoMIV & PstI<br>289.1 pSB1C3-mCherry: XbaI, PstI<br>297.1 pSBLB4B15-red (dam-): EcoRI & PstI<br><br><b>Ligation:</b><br>pSBLB4B15-CP11-fusionGFP (297.1+63.2+191.1)<br>pSBLB4B15-CP30-fusionGFP (297.1+65.1+191.1)   		 <br>pSBLB4B15-CP11-usp45-fusionGFP (297.1+63.2+usp45+191.1)   <br>pSBLB4B15-CP30-usp45-fusionGFP (297.1+65.2+usp45+191.1)    <br>pSBLB4B15-CP11-usp45-mCherry (297.1+63.2+usp45+289.1)   	 <br>pSBLB4B15-CP30-usp45-mCherry (297.1+65.2+usp45+289.1)   	 <br>pSB1C3-usp45 (1+usp45)<br>pSB1C3-usp45 (6+usp45)<br>pSBLb4C15-CP1-B0034-BFP<br>pSBLb4C15-CP8-B0034-BFP<br>pSBLb4C15-CP11-B0034-BFP<br>pSBLb4C15-CP29-B0034-BFP<br>pSBLb4C15-CP30-B0034-BFP<br>pSBLb4C15-CP41-B0034-BFP<br>pSBLb4C15-CP44-B0034-BFP<br>pSBLb4C15-CP11-aeblue<br>pSBLb4C15-CP30-aeblue<br>pSBLb4B15-CP11-mcherry<br>pSBLb4B15-CP30-mcherry<br>pSBLb4B15-CP11-tspurple<br>pSBLb4B15-CP30-tspurple<br>pSBLb4B15-CP11-amilCP<br>pSBLb4B15-CP30-amilCP<br>pSBLb4B15-CP11-amilGFP<br>pSBLb4B15-CP30-amilGFP<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>265.pSB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.pSB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br>303.pSB1A3-j23101-B0034-CrtZ<br>304.pSB1A3-j23106-B0034-CrtZ<br><br><b>Transformation:</b><br>108.pEL3S17-J23106-B0034-IspA<br>141.pRL3S17-J23101-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>272.pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303.pSB1A3-j23101-B0034-CrtZ<br>304.pSB1A3-j23106-B0034-CrtZ<br>204(1-4)<br> 	<br><b>Re-streak:</b><br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br><br><b>Screen/ ON:</b><br>219.pSB1C3-Miraculin<br>304.pSB1A3-J23106-B0034-CrtZ<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>280. pSB1A3-B0034-aeBlue<br>281. pSB1K3-J23110-B0034-aeBlue<br>282. pSB3K3-J23110-B0034-amilCP<br>283. pSB3K3-J23110-B0034-asCP <br><br><b>Plasmid prep. </b><br>110.pEL3S17-K206001-B0034-IspA<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br><br><b>Mutagenesis PCR:</b><br>336. pSB1C3-spisPink<br>337. pSB1C3-scOrange<br>338. pSB1C3-meffBlue<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>257. pSB1C3-pemp<br>288. pSB1C3-pem<br><br><h2>Other experiments:</h2><br><b>Phosphorylation of primers (20µl of each):</b><br>Pem-mutE_F<br>Pem-mutE_R<br>Pem-mutS_F<br>Pem-mutS_R<br><br><b>Cleaning of electroporation cuvettes</b></div>'
+ }
+      else if(id == 'd2013822')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-08-22</h1><br><b>Name of participants: </b>Anders Edlund, Anton Berglund, Mikael Strandgren, Viktor Blomkvist, Stephanie Herman, Alona Nyberg, Alexander, Pontus, Sabri, Malin, Magnus<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>257. pSB1C3-pemp<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>147.pSB1C3-B0034-Thioredoxin<br>184.pSB1C3-B0034-CrtZ<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>115. pSB3K5-J23101-B0034-His-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL (from 15)<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work:</h2><br><b>Competent cell preparation:</b><br>Lb11.1 plantarum 36E no plasmid<br>Lb12.1 reuteri DSM 20016 no plasmid<br>Lb12.2 reuteri DSM 20016 no plasmid<br><br><b>Digest:</b><br>147.B0034-Thioredoxin (E, S)<br>147.B0034-Thioredoxin (X,P)   <br>27.pEL3K16-red (E,P)<br>125.pSB1C3-ZCD-His (X,P)<br><br><b>Ligation:</b><br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><b>Transformation:</b><br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>Lb31. plantarum 36E pVS2<br>Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP<br>Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP<br>Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple<br>Lb35. reuteri DSM 20016 pVS2<br>Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry<br>Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP<br>Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP<br>Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP<br>Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue<br>Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue<br>Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP<br>Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP<br>Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP<br>Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP<br>Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP<br><strike>3.2. pVS2 (died in electroporation)<br>310. pSBLB4B15-CP11-fusionGFP (died in electroporation)<br>318. pSBLB4B15-CP11-mCherry (died in electroporation)<br>320. pSBLB4B15-CP11-amilGFP (died in electroporation)<br>313. pSBLB4B15-CP30-usp45-fusionGFP (died in electroporation)<br>317. pSBLB4B15-CP30-tsPurple (died in electroporation)<br>331. pSBLB4C15-CP41-BFP (died in electroporation)<br>332. pSBLB4C15-CP44-BFP (died in electroporation)</strike><br><b>+ other E. coli transformations</b><br><br><b>Mutagenesis PCR:</b><br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br><br><b>Screen/ ON:</b><br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1A3-j23101-B0034-CrtZ<br>304.pSB1A3-j23106-B0034-CrtZ<br><br><b>Plasmid prep. / Seq. prep.</b><br>147.pSB1C3-B0034-Thioredoxin<br>184.pSB1C3-B0034-CrtZ<br><br><b>Gel:</b><br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>207.CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><br><b>Phusion PCR:</b><br>->219.pSB1C3-Miraculin<br><br><h2>Results:</h2><br><b>Mutagenesis PCR:</b><br>257. pSB1C3-pemp: Problem with PCR-machine, paused at 98*C for 25 min. → re-do.<br>288. pSB1C3-pem:  				--”--<br><br><b>Transformation:</b><br>11.1 Lb. plantarum 36E:<br>3.2 pVS2: survived electroporation<br>3.2 pVS2: died during electroporation<br>310. pSBLB4B15-CP11-fusionGFP: died during electroporation<br>311. pSBLB4B15-CP30-fusionGFP: survived electroporation<br>312. pSBLB4B15-CP11-usp45-fusionGFP: survived electroporation<br>313. pSBLB4B15-CP30-usp45-fusionGFP: died during electroporation<br>316. pSBLB4B15-CP11-tsPurple: survived electroporation<br>317. pSBLB4B15-CP30-tsPurple: died during electroporation<br><br><b>12.2 Lb. reuteri DSM 20016:</b><br>3.2 pVS2: survived electroporation<br>318. pSBLB4B15-CP11-mCherry: died during electroporation<br>319. pSBLB4B15-CP30-mCherry: survived electroporation<br>320. pSBLB4B15-CP11-amilGFP: died during electroporation<br>321. pSBLB4B15-CP30-amilGFP: survived electroporation<br>322. pSBLB4B15-CP11-amilCP: survived electroporation<br>323. pSBLB4B15-CP30-amilCP: survived electroporation<br>324. pSBLB4C15-CP11-aeBlue: survived electroporation<br><br><b>12.1 Lb. reuteri DSM 20016:</b><br>325. pSBLB4C15-CP30-aeBlue: survived electroporation<br>326. pSBLB4C15-CP1-BFP: survived electroporation<br>327. pSBLB4C15-CP8-BFP: survived electroporation<br>328. pSBLB4C15-CP11-BFP: survived electroporation<br>329. pSBLB4C15-CP29-BFP: survived electroporation<br>330. pSBLB4C15-CP30-BFP: survived electroporation<br>331. pSBLB4C15-CP41-BFP: died during electroporation<br>332. pSBLB4C15-CP44-BFP: died during electroporation<br><br><h2>Characterization:</h2><br><b>Western blot analysis (SDS-page) on constructs:</b><br>115. pSB3K5-J23101-B0034-His-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL (from 15) (neg.contr)<br><br><h2>Other experiments:</h2><br><b>Phosphorylation of primers (60 µl of each):</b><br>Pem-mutE_F<br>Pem-mutE_R<br>Pem-mutS_F<br>Pem-mutS_R</div>'
+ }
+      else if(id == 'd2013823')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-08-23</h1><br><b>Name of participants: </b>Anton Berglund, Stephanie Herman, Viktor Berglund, Mikael Strandgren, Anders Edlund, Alexander, Pontus, Sabri, Malin, Magnus, Marcus H, Karl H, Hampus.E, Lovisa P, Kristoffer L, Emil M<br><br><h2>Ongoing constructs:</h2><br><b>E. coli (D5α):</b><br>111.7 pSBLb4C15-red<br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work:</h2><br><b>Gel electrophoresis:</b><br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br>111.7 pSBLb4C15-red (cut with E and P) (to get reference/examine cutsites)<br><br><b>Mutagenesis PCR:</b><br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br>Touchdown with Tm1+2 and Tm1+4.<br><br><b>Transformation:</b><br>102.pSB1C3-CrtE<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br><br><b>Re-streak:</b><br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br><br><b>Screening O/N:</b><br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>301. pEL3S17-J23110-B0034-Thioredoxin<br><br><b>O/N:</b><br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br><br><b>Subclone:</b><br>280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue<br>281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue<br>282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP<br>283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP<br><br><b>Glycerol stock Plasmidprep/sekvensprep:</b><br>108.pEL3S17-J23106-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>184.pSB1C3-B0034-CrtZ<br>299.pEL3S17-J23101-B0034-Thioredoxin<br><br><h2>Results:</h2><br><b>Gel electrophoresis:</b><br>257. pSB1C3-pemp with Pem-mutE_F & Pem-mutE_R: Unspecific binding, nc ok!*<br>288. pSB1C3-pem with Pem-mutE_F & Pem-mutE_R: Unspecific binding, nc ok!*<br>111.7 pSBLb4C15-red (cut with E and P): No band<br><i>	*→ redo mutagenesis PCR with higher temperatures</i></div>'
+ }
+      else if(id == 'd2013824')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-08-24<h1><br><b>Name of participants: </b>Mikael Strandgren, Anders Berglund, Alexander, Pontus, Sabri, Magnus, Marcus H, Karl H, Hampus.E, Lovisa P, Kristoffer L, Emil M<br><br><h2>Ongoing constructs:</h2><br><b>Escherichia coli</b><br>257. pSB1C3-pemp<br>288. pSB1C3-pem<br>136. pSB1C3-B0034-USP<br>pSB1C3-B0034-usp45<br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br><br><b>Lactobacillus reuteri:</b><br>Lb35. reuteri DSM 20016 pVS2<br>Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry<br>Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP<br>Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP<br>Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP<br>Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue<br>Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue<br>Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP<br>Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP<br>Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP<br>Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP<br>Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP<br><br><b>Lactobacillus plantarum:</b><br>Lb31. plantarum 36E pVS2<br>Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP<br>Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP<br>Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple<br><br><h2>Todays work:</h2><br><b>Mutagenesis</b><br>338.pSB1C3-meffBlue <br><br><b>Digest:</b><br>200.pSB1C3-B0034-asCP E,X<br>148.pSB1C3-RBS-gfasPurple E,X<br>20.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 E,X<br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R (EcoRI)<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R (EcoRI)<br><br><b>Ligation:</b><br>200.pSB1C3-B0034-asCP+91.BBa_J61002 (amp)-J23106→<br>→ 343.J23106-B0034-asCP<br>148.pSB1C3-RBS-gfasPurple+91.BBa_J61002 (amp)-J23106→<br>→ 160.j23106-RBS-gfasPurple<br>148.pSB1C3-RBS-gfasPurple+92.BBa_J61002 (amp)-J23110→<br>→ 159j23110-RBS-gfasPurple<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1+6.pSB1K3-red→<br>→215.pSB1K3-pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>184.pSB1C3-B0034-CrtZ+6.pSB1K3-Red+103.pSB1K3-J23101→<br>→ 303.pSB1K3-J23101-B0034-CrtZ<br>127.pSB1A3-B0034-UGTCs2-His+7.pSB3K3-Red+CP8→<br>→ 223.pSB3K3-CP8-B0034-UGTCs2-His<br><br><b>Transformation:</b><br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>343.pSB1C3-J23106-B0034-asCP<br>160.pSB1C3-j23106-RBS-gfasPurple<br>159.pSB1C3-j23110-RBS-gfasPurple<br>303.pSB1K3-j23101-B0034-CrtZ<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>336.pSB1C3.spisPink<br>337.pSB1C3.scOrange<br>284.pSB1C3.B0034-aeBlue<br>285.pSB1C3.J23110-B0034-aeBlue<br>286.pSB1C3.J23110-B0034-amilCP<br>287.pSB1C3-J23110-B0034-asCP<br>336.pSB1C3-spisPink<br>337.pSB1C3-scOrange<br>284. pSB1C3-B0034-aeBlue<br>285. pSB1C3-J23110-B0034-aeBlue<br>286. pSB1C3-J23110-B0034-amilCP<br>287. pSB1C3-J23110-B0034-asCP<br><br><b>O/N screening:</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>Lb31. plantarum 36E pVS2<br>Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP<br>Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP<br>Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple<br>Lb35. reuteri DSM 20016 pVS2<br>Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry<br>Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP<br>Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP<br>Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP<br>Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue<br>Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue<br>Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP<br>Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP<br>Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP<br>Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP<br>Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP<br>136.<br>312<br>313<br>315<br>321<br>325<br>327<br>332<br>317<br><br><b>O/N:</b><br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>143. pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>265. J23110-B0034-Exp-Linker-Miraculin-His<br>301. pEL3S17-J23110-B0034-Thioredoxin<br><br><b>Plasmid prepp & sequence prepp</b><br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br><br><b>Gel electrophoresis:</b><br>Lb31. plantarum 36E pVS2<br>Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP<br>Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP<br>Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple<br>Lb35. reuteri DSM 20016 pVS2<br>Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry<br>Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP<br>Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP<br>Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP<br>Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue<br>Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue<br>Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP<br>Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP<br>Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP<br>Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP<br>Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP<br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br><i>+ E. coli transformations</i><br><br><b>PCR-purification:</b><br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br><br><b>DpnI:</b><br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br><br><h2>Results:</h2><br><b>Transformations:</b><br>All transformations spread on Bleomycin/zeocin have grown! If they are the right lactobacilli with the right plasmid remains to be seen, there seem to be a lot of contamination in the form of “clouds” covering all plates. Yet still there are larger colonies that very well could be what we want. Maybe the antibiotics are not efficient enough, we will try to spread normal plasmid free reuteri from frozen stock on the plates to investigate… We’ll also try a colony screening today with VF2 and VR. <br>Nothing grew on chloramphenicol plates, not even the positive control pVS2, though it grew on erythromycin both when transformed into reuteri and plantarum. <br><br><b>Gel electrophoresis:</b><br>All Pem/Pemp mutagenesises showed bands at the right lengths (~2700 bp), although they were a bit smudgy - weakly stretching backward toward the wells. The digests of Pem simply showed one band, a weaker counterpart of the uncut PCR-product. We suspect the digest didn’t go as supposed, maybe due to the PCR buffer. <br>The lactobacillus colony screenings showed up empty, perhaps the cells didn’t lyse? Or didn’t contain plasmids with VF and VR… We will continue to investigate the resistance problems, and await the sequencing results of the insertsite.<br>The screenings of e. coli transformations done thursday also gave us an empty gel, without even ladders. Possibly the gel had to shallow wells.</div>'
+ }
+      else if(id == 'd2013825')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday 2013-08-25</h1><br><b>Name of participants:</b> Alexander, Pontus, Sabri, Malin, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs:</h2><br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work:</h2><br><b>Mutagenesis</b><br>338.pSB1C3-meffBlue<br><br><b>Plasmidprepp:</b><br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>265.pSB3K3.J23110-B0034-Exp-Linker-Miraculin-His<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>143. pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>265. J23110-B0034-Exp-Linker-Miraculin-His<br>301. pEL3S17-J23110-B0034-Thioredoxin<br><br><b>Digest:</b><br>147.pSB1C3-B0034-Thioredoxin<br><br><b>Ligate:</b><br>147.pSB1C3-B0034-Thioredoxin+CP1→<br>→ 346.pSB1C3-CP1-B0034-Thioredoxin<br>147.pSB1C3-B0034-Thioredoxin+CP8→<br>→347.pSB1C3-CP8-B0034-Thioredoxin<br>147.pSB1C3-B0034-Thioredoxin+CP41→<br>→348.pSB1C3-CP41-B0034-Thioredoxin<br><br><b>Transformation:</b><br>1.pSB1C3-Red (competens test)<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>285.pSB1C3.J23110-B0034-aeBlue<br>286.pSB1C3.J23110-B0034-amilCP<br>287.pSB1C3-J23110-B0034-asCP303.pSB1K3-j23101-B0034-CrtZ<br>336.pSB1C3-spisPink<br>337pSB1C3-scOrange<br>346.pSB1C3-CP1-B0034-Thioredoxin<br>347.pSB1C3-CP8-B0034-Thioredoxin<br>348.pSB1C3-CP41-B0034-Thioredoxin<br>285. pSB1C3-J23110-B0034-aeBlue<br>286. pSB1C3-J23110-B0034-amilCP<br>287. pSB1C3-J23110-B0034-asCP<br>336.pSB1C3-spisPink<br>337.pSB1C3-scOrange<br>159. pSB1C3-J23110-RBS-gfasPurple<br>160. pSB1C3-J23110-RBS-gfasPurple<br><br><b>Re-streak:</b><br>284. pSB1C3-B0034-aeBlue<br><br><b>Sequenceprep:</b><br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br></div>'
+ }
   else if(id == 'd2013919')
   {

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