Team:MSOE Milwaukee/Primers

From 2013.igem.org

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<TABLE>
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   <TD bgcolor = #649327 width = 320 valign = bottom height = 50><font color = "white" size = "+20">GENE</TD>
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   <TD bgcolor = #649327 width = 200 valign = bottom height = 50><font color = "white" size = "+20">PRIMER</TD>
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   <TD bgcolor = #649327 width = 320 valign = bottom height = 50><font color = "white" size = "+20">ENZYME</TD>
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   <TD bgcolor = #649327 width = 760 valign = bottom height = 50><font color = "white" size = "+20">SEQUENCE (5' to 3')</TD>
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  <TD bgcolor = #649327 width = 320 valign = bottom height = 50><font color = "white" size = "+20">SIZE</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">xynC-A</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1,8-cineole synthase forward</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">PURPOSE</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">ATCGGAATTCGCGGCCGCTTCTAGATGGCTACCCTGCGTATC</TD>
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  <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">594</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">xyloA</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1,8-cineole synthase reverse</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">PURPOSE</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">GCTAATGATCATCGCCGGCGACGTCCTTATTAACCCAGACGGTTAACG</TD>
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  <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1020</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">mvaK2</TD>
   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">mvaK2</TD>
   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">Phosphomevalonate kinase</TD>
   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">Phosphomevalonate kinase</TD>
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  <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1011</TD>
 
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">mvaD</TD>
   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">mvaD</TD>
   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">Mevalonate-5-pyrophosphate decarboxylase</TD>
   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">Mevalonate-5-pyrophosphate decarboxylase</TD>
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  <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">954</TD>
 
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">TPS-CIN</TD>
   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">TPS-CIN</TD>
   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1,8-cineole synthase</TD>
   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1,8-cineole synthase</TD>
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  <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1800</TD>
 
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Revision as of 18:24, 8 July 2013

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PRIMER SEQUENCE (5' to 3')
1,8-cineole synthase forward ATCGGAATTCGCGGCCGCTTCTAGATGGCTACCCTGCGTATC
1,8-cineole synthase reverse GCTAATGATCATCGCCGGCGACGTCCTTATTAACCCAGACGGTTAACG
mvaS HMG-CoA synthase 1152
mvaK1 Mevalonate Kinase 879
mvaK2 Phosphomevalonate kinase
mvaD Mevalonate-5-pyrophosphate decarboxylase
TPS-CIN 1,8-cineole synthase