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Team:MSOE Milwaukee/Primers
From 2013.igem.org
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| - | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1,8-cineole synthase | + | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1,8-cineole synthase</TD> |
<TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white">ATCGGAATTCGCGGCCGCTTCTAGATGGCTACCCTGCGTATC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAACCCAGACGGTTAACG</TD> | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white">ATCGGAATTCGCGGCCGCTTCTAGATGGCTACCCTGCGTATC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAACCCAGACGGTTAACG</TD> | ||
</TR> | </TR> | ||
<TR align = center> | <TR align = center> | ||
| - | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2"> | + | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">HMG-CoA Synthase reverse</TD> |
| - | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" > | + | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGACCATCGGTATCGAC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAGTTACGGTAAGAACGAACG</TD> |
</TR> | </TR> | ||
<TR align = center> | <TR align = center> | ||
| - | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2"> | + | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">Mevalonate Kinase</TD> |
| - | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white"> | + | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white">ATCGGAATTCGCGGCCGCTTCTAGCATGACCAAAAAAGTTGGTGTTGG<br>GCTAATGATCATCGCCGGCGACGTCCTTATTACAGAGATTCGATCCAGG</TD> |
</TR> | </TR> | ||
<TR align = center> | <TR align = center> | ||
| - | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2"> | + | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">Phosphomevalonate Kinase</TD> |
| - | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" > | + | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGATCGCTGTTAAAACCTGC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAAGATTTGTCGTCGTGACC</TD> |
</TR> | </TR> | ||
<TR align = center> | <TR align = center> | ||
| - | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2"> | + | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">Mevalonate Diphosphate Kinase</TD> |
| - | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2"> | + | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">ATCGGAATTCGCGGCCGCTTCTAGCATGGACCGTGAACCGG<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAGCAGCAGTCGTCC</TD> |
</TR> | </TR> | ||
<TR align = center> | <TR align = center> | ||
| - | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2"> | + | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">Xylanase C</TD> |
| - | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2"> | + | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">Xylanase C ATCGGAATTCGCGGCCGCTTCTAGCATGTTCTGCTCTAACGCTCAGC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAGGTCAGACCCATTTTTTCC</TD> |
</TR> | </TR> | ||
<TR align = center> | <TR align = center> | ||
| - | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2"> | + | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">Xylosidase</TD> |
| - | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2"> | + | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">ATCGGAATTCGCGGCCGCTTCTAGCATGAAATCTAAACTGCTGTTCCCG<br>GCTAATGATCATCGCCGGCGACGTCTTATTACGGTTTTTTGGTCAGG</TD> |
| + | </TR> | ||
| + | <TR align = center> | ||
| + | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">osmY with GS liner (two reverse)</TD> | ||
| + | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">ATCGGAATTCGCGGCCCGCTTCTAGATGACTATGACAAGACTGAAG<br>GGATCCCCCGCTACCACTGCCCGAACCCTTAGTTTTCAGATCATT<br>GCTAATGATCATCGCCGGCGACGTCGGATCCCCCGCTACCACTGCCC</TD> | ||
</TR> | </TR> | ||
</TABLE> | </TABLE> | ||
</HTML> | </HTML> | ||
Revision as of 18:43, 8 July 2013
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| DNA SEQUENCE/ENZYME | PRIMER TO ADD THE PREFIX (FOR) 5' to 3' PRIMER TO ADD THE SUFFIX (REV) 5' to 3' |
| 1,8-cineole synthase | ATCGGAATTCGCGGCCGCTTCTAGATGGCTACCCTGCGTATC GCTAATGATCATCGCCGGCGACGTCCTTATTAACCCAGACGGTTAACG |
| HMG-CoA Synthase reverse | ATCGGAATTCGCGGCCGCTTCTAGCATGACCATCGGTATCGAC GCTAATGATCATCGCCGGCGACGTCCTTATTAGTTACGGTAAGAACGAACG |
| Mevalonate Kinase | ATCGGAATTCGCGGCCGCTTCTAGCATGACCAAAAAAGTTGGTGTTGG GCTAATGATCATCGCCGGCGACGTCCTTATTACAGAGATTCGATCCAGG |
| Phosphomevalonate Kinase | ATCGGAATTCGCGGCCGCTTCTAGCATGATCGCTGTTAAAACCTGC GCTAATGATCATCGCCGGCGACGTCCTTATTAAGATTTGTCGTCGTGACC |
| Mevalonate Diphosphate Kinase | ATCGGAATTCGCGGCCGCTTCTAGCATGGACCGTGAACCGG GCTAATGATCATCGCCGGCGACGTCCTTATTAGCAGCAGTCGTCC |
| Xylanase C | Xylanase C ATCGGAATTCGCGGCCGCTTCTAGCATGTTCTGCTCTAACGCTCAGC GCTAATGATCATCGCCGGCGACGTCCTTATTAGGTCAGACCCATTTTTTCC |
| Xylosidase | ATCGGAATTCGCGGCCGCTTCTAGCATGAAATCTAAACTGCTGTTCCCG GCTAATGATCATCGCCGGCGACGTCTTATTACGGTTTTTTGGTCAGG |
| osmY with GS liner (two reverse) | ATCGGAATTCGCGGCCCGCTTCTAGATGACTATGACAAGACTGAAG GGATCCCCCGCTACCACTGCCCGAACCCTTAGTTTTCAGATCATT GCTAATGATCATCGCCGGCGACGTCGGATCCCCCGCTACCACTGCCC |
