Density Control

The final aim is to control our bacterial population with only one data available : the global fluorescence. But in the experiments, in order to prove that our system works, besides the fluorescence we measured the $OD_{600}$ and the amount of living cells.

At first, we wanted to have a constant population of living bacteria, therefore an $OD_{600}$ going up in a linear way. The model was necessary to find what would be the best illumination to provide to have as quickly as possible a stable level of the amount of living bacteria.

Proof of Concept

Model Predictive Control

Method to Control a Bacterial Population

When the model's parameters were calculated, it appeared that some of them ($b$, $k$ and $l$) were very variable, their value depended a lot on the preparation of the experiment. Sadly, it is important that they are the more accurate possible, the quality of the predictions depend greatly on them. That is the reason why those 3 parameters shall be slightly modified for each experiment.