Team:UNITN-Trento/Notebook/Labposts/06/01

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{
{
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"date" : "2013-06-10",
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"date" : "2013-06-05",
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"author" : "michele-emil",
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"author" : "viola",
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"title" : "Transformation of the product of ligation SAMsynthetase + psB1C3.",
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"title" : "transformation buffer",
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"content" : "<html>We took the ligation products (SAMsynthetase + pSB1C3 in two different rapports 2:1 and 1:!) done by Caterina and Fabio in the morning and we transformed it in NEB10 cells. We've also prepared some inocula from the plates of the devices extract in CiBio (BBa_J45700, BBa_J45320, BBa_J45119)in LB with the correct antibioticum to have a lot of materials to work on.</html>",
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"content" : "<html>I made the transformation buffer for the competent cells that we will do tomorrow, 10mM tris HCl and 50 mM CaCl2. </html>",
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"tags" : "SAMsynthetase-PchB-PchBA-BSMT1"
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"tags" : "competent cells"
}
}

Revision as of 07:38, 3 October 2013

{ "date" : "2013-06-05", "author" : "viola", "title" : "transformation buffer", "content" : "I made the transformation buffer for the competent cells that we will do tomorrow, 10mM tris HCl and 50 mM CaCl2. ", "tags" : "competent cells" }

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