Team:UNITN-Trento/Notebook/Labposts/06/02

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(Difference between revisions)
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{
{
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"date" : "2013-06-12",
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"date" : "2013-06-06",
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"author" : "caterina-michele",
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"author" : "viola-bruno",
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"title" : "miniprep SAMsynthetase+pSB1C3!",
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"title" : "Transformations of the LMU Munich plasmids for B.subtilis",
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"content" : "We took the three inocula of <b>SAMsynthetase+pSB1C3</b> and we performed the purification (Wizard Plus SV Miniprep DNA purification system). Then to verify if the colonies that we took contain the correct insert we perform the screening protocol and the gel eletrophoresis.",
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"content" : "Me and Bruno transformed the parts that LMU Munich sent us in NEB10beta cells line.The following transformation worked:Bba_K823022 (pSBBS4S)Bba_K823027 (pSBBS2E)Bba_K823023 (pSBBS1C)",
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"tags" : "SAMsynthetase"
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"tags" : "pSBBs1C-pSBBs4S-pSBBs2E"
}
}

Revision as of 07:38, 3 October 2013

{ "date" : "2013-06-06", "author" : "viola-bruno", "title" : "Transformations of the LMU Munich plasmids for B.subtilis", "content" : "Me and Bruno transformed the parts that LMU Munich sent us in NEB10beta cells line.The following transformation worked:Bba_K823022 (pSBBS4S)Bba_K823027 (pSBBS2E)Bba_K823023 (pSBBS1C)", "tags" : "pSBBs1C-pSBBs4S-pSBBs2E" }

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