Team:UNITN-Trento/Notebook/Labposts/06/03

From 2013.igem.org

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{
{
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"date" : "2013-06-12",
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"date" : "2013-06-06",
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"author" : "viola-thomas",
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"author" : "bruno",
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"title" : "SAMsynthetase + pSB1C3 (linearized or with RFP) digestion and purification ",
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"title" : "NEB10beta competent Cells",
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"content" : "We digested using XbaI and PstI and purified the products previously obtained via PCR and via extraction from the registry (pSB1C3 with RFP). We then quantified them at the nano-drop. {{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification results|<html><center><table class=\"tn-sp-table\"><tr><th>pSB1C3 + RfP</th><td>27,8 ng/ul</td></tr><tr><th>pSB1C3 linearized</th><td>17,7 ng/ul</td></tr><tr><th>SAM Synthetase</th><td>13,2 ng/ul</td></tr></table></center></html>}}",
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"content" : "<html>I made competent cells from the stock of NEB10beta. I followed the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols\">protocol</a></html>",
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"tags" : "SAMsynthetase"
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"tags" : "mix"
}
}

Revision as of 07:39, 3 October 2013

{ "date" : "2013-06-06", "author" : "bruno", "title" : "NEB10beta competent Cells", "content" : "I made competent cells from the stock of NEB10beta. I followed the protocol", "tags" : "mix" }

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