Team:UNITN-Trento/Notebook/Labposts/06/09

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{
{
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"date" : "2013-06-26",
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"date" : "2013-06-07",
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"author" : "viola",
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"author" : "gabriele-michele",
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"title" : "Adding the terminator to EFE: day 3",
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"title" : "Third SAMsynthetase synthase extraction attempt - TOO SAD",
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"content" : "<html>i miniprepped the inocula from the transformation of B0015 both in psb1c3 and psb1ak3 and i made a 2% gel for screening the little terminator. I loaded the samples without dye and with 30% glycerol and this is the picture of the gel :</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/3/37/Tn-2013_Corsa20minPCR_B0015.jpg\" width=\"450px\" /></center></html>}}<html> on the left of the central 100bp ladder ther is B0015 in psb1ak3 and on the right there is B0015 in psb1c3.</html>",
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"content" : " We've tried another combination to maximize the outputs of the PCR done to amplify the SAMsynthetase synthase gene from an extract of E.coli genomic DNA (strain MG1655). This time we used the <I> RBC Taq DNA Polymerase </I> protocol but with a mix of this polymerase (0.25 ul) and the Phusion polymerase (0.3 ul). We've prepared two identical samples to see who is the better PCR maker! :) {{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel Image|<html><img src=\"https://static.igem.org/mediawiki/2013/c/c2/Tn-20130607-SAMsynth_pcr_gire-pedro.jpg\" /></html>}}As you can see from the picture we both obtained great results",
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"tags" : "EFE-B0015"
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"tags" : "SAMsynthetase"
}
}

Revision as of 07:40, 3 October 2013

{ "date" : "2013-06-07", "author" : "gabriele-michele", "title" : "Third SAMsynthetase synthase extraction attempt - TOO SAD", "content" : " We've tried another combination to maximize the outputs of the PCR done to amplify the SAMsynthetase synthase gene from an extract of E.coli genomic DNA (strain MG1655). This time we used the RBC Taq DNA Polymerase protocol but with a mix of this polymerase (0.25 ul) and the Phusion polymerase (0.3 ul). We've prepared two identical samples to see who is the better PCR maker! :)

Gel Image
As you can see from the picture we both obtained great results", "tags" : "SAMsynthetase" }

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