Team:UNITN-Trento/Notebook/Labposts/06/11

From 2013.igem.org

(Difference between revisions)
 
Line 1: Line 1:
{
{
-
"date" : "2013-06-12",
+
"date" : "2013-06-10",
-
"author" : "emil-fabio",
+
"author" : "caterina-fabio",
-
"title" : "Purification and verification of three Bacillus backbonesg",
+
"title" : "The Registry 2013 is deflowered!",
-
"content" : "We have done the miniprep of the three backbone exploiting the Kit from Promega.N.B.Some sample have shown an unexpected red color. There is the possibilities that some backbones contain the RFP, maybe all. We have quantified the products with the following results:BBa_K823023(4 sample):220 ng/<html>&mu;</html>l , 228.9 ng/<html>&mu;</html>l ,246.4 ng/<html>&mu;</html>l , 272.3 ng/<html>&mu;</html>lBBa_K823022(3 sample): 222.8 ng/<html>&mu;</html>l , 227.5 ng/<html>&mu;</html>l ,284.9 ng/<html>&mu;</html>lBBa_K823027(2 sample):268 ng/<html>&mu;</html>l , 299.3 ng/<html>&mu;</html>lThen we have tried to verify the parts digesting with EcoR1 and Pst1 and doing an electrophoresis, unfortunately it seems that we didn't load the marker Fermentas 1 KB.",
+
"content" : "We extracted <b>BBa_K143012</b>, <b>BBa_K823000</b>, <b>BBa_K823002</b> and <b>BBa_K823003</b> and then transformed them into NEB10&beta; cells.",
-
"tags" : "pSBBs1C-pSBBs4S-pSBBs2E"
+
"tags" : "Pveg-PliaG-PlepA"
}
}

Latest revision as of 07:41, 3 October 2013

{ "date" : "2013-06-10", "author" : "caterina-fabio", "title" : "The Registry 2013 is deflowered!", "content" : "We extracted BBa_K143012, BBa_K823000, BBa_K823002 and BBa_K823003 and then transformed them into NEB10β cells.", "tags" : "Pveg-PliaG-PlepA" }

Retrieved from "http://2013.igem.org/Team:UNITN-Trento/Notebook/Labposts/06/11"