Team:UNITN-Trento/Notebook/Labposts/06/12

From 2013.igem.org

(Difference between revisions)
 
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{
{
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"date" : "2013-06-13",
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"date" : "2013-06-10",
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"author" : "Emil",
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"author" : "fabio-caterina",
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"title" : "Verfying the three backbone",
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"title" : "Purification of SAMsynthetase and pSB1C3.",
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"content" : "<html>We have verifyed the parts from LMU Munich doing an electrophoresis after having digested the parts with Pst1 and EcoR1.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel Results|<html><center><table class=\"tn-sp-table\"><tr><th>Well</th><th colspan=\"10\">Loaded samples</th></tr><tr><td>1</td><td>DNA Ladder 1Kb</td></tr><tr><td>2</td><td>#1BBa_J823022(A)</td></tr><tr><td>3</td><td>#2BBa_J823023(A)</td></tr><tr><td>4</td><td>#4BBa_J823027(A)</td></tr><tr><td>5</td><td>#5BBa_J823022(B)</td></tr><tr><td>6</td><td>#6BBa_J823023(B)</td></tr><tr><td>7</td><td>#7Bba_J823027(B)</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/b/be/Tn-20130613-Gel_LMU_Munich.jpg\" width=\"400px\" /></center></html>}}<html></html>",
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"content" : "<html>We purified the products of SAMsynthetase and pSB1C3 PCR performed by Gire and Pedro following the illustra™ GFX™ PCR DNA and Gel Band Purification Kit protocol. After the quantification with NanoDrop, we did the ligation reaction.</html>",
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"tags" : "pSBBs1C-pSBBs4S-pSBBs2E"
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"tags" : "SAMsynthetase"
}
}

Latest revision as of 07:41, 3 October 2013

{ "date" : "2013-06-10", "author" : "fabio-caterina", "title" : "Purification of SAMsynthetase and pSB1C3.", "content" : "We purified the products of SAMsynthetase and pSB1C3 PCR performed by Gire and Pedro following the illustra™ GFX™ PCR DNA and Gel Band Purification Kit protocol. After the quantification with NanoDrop, we did the ligation reaction.", "tags" : "SAMsynthetase" }