Team:UNITN-Trento/Notebook/Labposts/06/14

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(Difference between revisions)

Latest revision as of 07:42, 3 October 2013

{ "date" : "2013-06-11", "author" : "thomas-emil-michele", "title" : "Purification, quantification and digestion of SAMsynthetase and pSB1C3", "content" : "We purified the PCR (third attempt) products of SAMsynthetase and pSB1C3 linearized vector following the Quick Reference protocol. After that, we quantified them using the nanodrop instrument.

Quantification results

Sample	SAM Synthetase	pSB1C3
1	18,6 ng/ul	21,6 ng/ul
2	16,2 ng/ul	16 ng/ul

That's definetely not a good result, however we will continue working with them.", "tags" : "SAMsynthetase" }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-08-05",
+	"date" : "2013-06-11",
-	"author" : "viola",
+	"author" : "thomas-emil-michele",
-	"title" : "2nd ligation",
+	"title" : "Purification, quantification and digestion of SAMsynthetase and pSB1C3",
-	"content" : "After the negative screening of Pedro's inocula of the ligation of EFE + s04617 today I retried this cloning.",
+	"content" : "We purified the PCR (third attempt) products of SAMsynthetase and pSB1C3 linearized vector following the Quick Reference protocol. After that, we quantified them using the nanodrop instrument. {{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification results|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>SAM Synthetase</th><th>pSB1C3</th></tr><tr><td>1</td><td>18,6 ng/ul</td><td>21,6 ng/ul</td></tr><tr><td>2</td><td>16,2 ng/ul</td><td>16 ng/ul</td></table></center></html>}}That's definetely not a good result, however we will continue working with them.",
-	"tags" : "s04617-EFE"
+	"tags" : "SAMsynthetase"
 }