Team:UNITN-Trento/Notebook/Labposts/06/18

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(Difference between revisions)
 
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{
{
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"date" : "2013-06-06",
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"date" : "2013-06-12",
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"author" : "viola-bruno",
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"author" : "viola-thomas",
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"title" : "Transformations of the LMU Munich plasmids for B.subtilis",
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"title" : "SAMsynthetase + pSB1C3 (linearized or with RFP) digestion and purification ",
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"content" : "Me and Bruno transformed the parts that LMU Munich sent us in NEB10beta cells line.The following transformation worked:Bba_K823022 (pSBBS4S)Bba_K823027 (pSBBS2E)Bba_K823023 (pSBBS1C)",
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"content" : "We digested using XbaI and PstI and purified the products previously obtained via PCR and via extraction from the registry (pSB1C3 with RFP). We then quantified them at the nano-drop. {{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification results|<html><center><table class=\"tn-sp-table\"><tr><th>pSB1C3 + RfP</th><td>27,8 ng/ul</td></tr><tr><th>pSB1C3 linearized</th><td>17,7 ng/ul</td></tr><tr><th>SAM Synthetase</th><td>13,2 ng/ul</td></tr></table></center></html>}}",
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"tags" : "pSBBs1C-pSBBs4S-pSBBs2E"
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"tags" : "SAMsynthetase"
}
}

Latest revision as of 07:43, 3 October 2013

{ "date" : "2013-06-12", "author" : "viola-thomas", "title" : "SAMsynthetase + pSB1C3 (linearized or with RFP) digestion and purification ", "content" : "We digested using XbaI and PstI and purified the products previously obtained via PCR and via extraction from the registry (pSB1C3 with RFP). We then quantified them at the nano-drop.

Quantification results
pSB1C3 + RfP27,8 ng/ul
pSB1C3 linearized17,7 ng/ul
SAM Synthetase13,2 ng/ul
", "tags" : "SAMsynthetase" }

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