Team:UNITN-Trento/Notebook/Labposts/06/19

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(Difference between revisions)
 
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{
{
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"date" : "2013-06-13",
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"date" : "2013-06-12",
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"author" : "viola-bruno",
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"author" : "thomas-viola",
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"title" : "Transformation efficiency kit",
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"title" : "Transformation of four B.subtilis promoters",
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"content" : "We followed the protocol for the transformation efficiency kit to determine how efficiency are our competent cells. We performed 5 transformations with 5 different concetrations of the part Bba_J04450 in the plasmid pSB1C3. ",
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"content" : "Due to a too high concentration of Cloramphenicol in the plates, we have to re-transform the promoters previously extracted from the registry. We transformed and plated the following parts:- K323002- K143012- K323000- K323003'''Results:''' since we didn't obtain any colonies we failed the experiment.",
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"tags" : "RFP"
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"tags" : "Pveg-PliaG-PlepA"
}
}

Latest revision as of 07:43, 3 October 2013

{ "date" : "2013-06-12", "author" : "thomas-viola", "title" : "Transformation of four B.subtilis promoters", "content" : "Due to a too high concentration of Cloramphenicol in the plates, we have to re-transform the promoters previously extracted from the registry. We transformed and plated the following parts:- K323002- K143012- K323000- K323003Results: since we didn't obtain any colonies we failed the experiment.", "tags" : "Pveg-PliaG-PlepA" }

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