Team:UNITN-Trento/Notebook/Labposts/06/48

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(Difference between revisions)
 
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{
{
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"date" : "2013-06-24",
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"date" : "2013-06-22",
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"author" : "emil-gabriele",
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"author" : "thomas",
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"title" : "Inocula of R0010",
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"title" : "Minipreps and screening of AraCpBAD + EFE (in pSB1C3)",
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"content" : "<html>We performed 5 inocula from the plate containing the colonies resulted from the transformation of R0010 (in pSB1A2).<br/>The LB medium contained Ampicillin (1:1000).</html>",
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"content" : "<html>Starting from the inocula of yesterday, I did minipreps following <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#miniprep\">this protocol</a>.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Pellets Image|<html><center><img id=\"post_img\" src=\"https://static.igem.org/mediawiki/2013/3/36/Tn-20130622-Pellet_TP.jpg\" width=\"450px\" /></center></html>}}<html></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification results|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>ng/ul</th></tr><tr><td>1:1 Plate1 n°1</td><td>1201,5</td></tr><tr><td>1:1 Plate1 n°2</td><td>1620,5</td></tr><tr><td>1:2 Plate1 n°1</td><td>969,8</td></tr><tr><td>1:4 Plate1 n°1</td><td>636,3</td></tr><tr><td>1:1 Plate2 n°1</td><td>672,8</td></tr><tr><td>1:1 Plate2 n°2</td><td>1222,5</td></tr><tr><td>1:2 Plate2 n°1</td><td>782,0</td></tr><tr><td>1:4 Plate2 n°1</td><td>705,3</td></tr></table></center></html>}}<html>As you can see we obtained a set of very high concentrations. We proceeded then with the screening test. To do that we digested 800 ng of DNA with EcorI and PstI following <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion\">the screening digestion protocol</a>. In the end we prepared the sample for an electrophoresis analysis.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel Image|<html><img id=\"post_img\" src=\"https://static.igem.org/mediawiki/2013/8/8b/Tn-20130622-Thomas_AraCpBAD_EFE.jpg\" width=\"450px\"/></html>}}<html> <br/> As you can see, 6 samples out of 8 have the expected bands: 2070bp for the vector and 2300bp for AraCpBAD.</html>",
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"tags" : "Plac"
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"tags" : "EFE-AraCpBAD"
}
}

Latest revision as of 07:54, 3 October 2013

{ "date" : "2013-06-22", "author" : "thomas", "title" : "Minipreps and screening of AraCpBAD + EFE (in pSB1C3)", "content" : "Starting from the inocula of yesterday, I did minipreps following this protocol.

Pellets Image
Quantification results
Sampleng/ul
1:1 Plate1 n°11201,5
1:1 Plate1 n°21620,5
1:2 Plate1 n°1969,8
1:4 Plate1 n°1636,3
1:1 Plate2 n°1672,8
1:1 Plate2 n°21222,5
1:2 Plate2 n°1782,0
1:4 Plate2 n°1705,3
As you can see we obtained a set of very high concentrations. We proceeded then with the screening test. To do that we digested 800 ng of DNA with EcorI and PstI following the screening digestion protocol. In the end we prepared the sample for an electrophoresis analysis.
Gel Image

As you can see, 6 samples out of 8 have the expected bands: 2070bp for the vector and 2300bp for AraCpBAD.", "tags" : "EFE-AraCpBAD" }

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