Team:UNITN-Trento/Notebook/Labposts/06/50

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(Created page with "{ "date" : "2013-06-25", "author" : "caterina-michele", "title" : "The final device: BBa_J45320(placI and PchBA)+ BBa_K1065101(araC-pBAD promoter and BMST1) in pSB1C3", "cont...")
 
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{
{
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"date" : "2013-06-25",
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"date" : "2013-06-24",
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"author" : "caterina-michele",
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"author" : "viola",
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"title" : "The final device: BBa_J45320(placI and PchBA)+ BBa_K1065101(araC-pBAD promoter and BMST1) in pSB1C3",
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"title" : "Adding the terminator to EFE: day 1",
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"content" : " We performed the digestion and the ligation on:1a)BBa_K1065101 with EcoR I and Xba I and1b)BBa_J45320 with EcoR I and Spt I to obtain the complete device BBa_K1065102(placI+PchBA+araC-pBAD promoter+BMST1 in pSB1C3)2a)pSB1C3 linearized with EcoR I and Pst I2b)BBa_J45320 with EcoR I and Pst Ito obtain the device BBa_K1065103 (placI+PchBA in pSB1C3).At the end of the day we did the trasformaion in NEB10β cells. Waiting for the results...break a leg! ",
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"content" : "<html>Today i started the short cloning protocol to add the double terminator B0015 at the end of EFE. First i extracted the parts from the registry from the plate 3 well 4F (the terminator in psb1c3) and from the plate 5 well 23L (in psb1ak3).   </html>",
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"tags" : "PchBA-BMST1-araC-pBAD"
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"tags" : "EFE-B0015"
}
}

Latest revision as of 07:55, 3 October 2013

{ "date" : "2013-06-24", "author" : "viola", "title" : "Adding the terminator to EFE: day 1", "content" : "Today i started the short cloning protocol to add the double terminator B0015 at the end of EFE. First i extracted the parts from the registry from the plate 3 well 4F (the terminator in psb1c3) and from the plate 5 well 23L (in psb1ak3). ", "tags" : "EFE-B0015" }

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