Team:UNITN-Trento/Notebook/Labposts/06/61

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(Difference between revisions)

Latest revision as of 07:58, 3 October 2013

{ "date" : "2013-06-26", "author" : "caterina-michele", "title" : "Don't Trust few colonies!!!", "content" : " The results of the transformations were not so good. We've only few colonies on each plate (only on Michele's control there were many colonies!). Despite this, we've decided to do inocula of some colonies in the afternoon. On the rest of the day Michele tried two different PCRs to obtain pSB1C3 linearized (someone stole his supplies!!). Obviously no results (immagini gel?). Caterina repeated the digstion but she used two differents type of ligase for the reaction of ligation. At the end of the day she transformed Nebα with these new products of ligation. ", "tags" : "PchBA-BMST1-araC-pBAD" }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-06-20",
+	"date" : "2013-06-26",
-	"author" : "fabio-bruno",
+	"author" : "caterina-michele",
-	"title" : "bacillus subtilis promoters and  plasmids (part 5)!!",
+	"title" : "Don't Trust few colonies!!!",
-	"content" : "<html> OOPS… I DID IT AGAIN!!! Yesterday’s screening was a total failure, so today we tried to screen K823000a and k143012b but unfortunately we chocked again, twice!! We made 2 different gels, with 2 different formulas (the first one with 1 % of agarose, the second one with 1,5 % and 100bp ladder, right for short parts). At the same time we failed to screen a bunch of new promoters miniprepped this morning from some overnight inocula: K823003, k823002, k143012 (all transformed in NEB5a)While attempting to screen those parts we transformed and plated NEB5a with k823000; K050901; K050904 and two new backbones for B.subtilis: K823024 (Pxil) and K823026 (Pspac).</html>",
+	"content" : " The results of the transformations were not so good. We've only few colonies on each plate (only on Michele's control there were many colonies!). Despite this, we've decided to do inocula of some colonies in the afternoon. On the rest of the day Michele tried two different PCRs to obtain pSB1C3 linearized (someone stole his supplies!!). Obviously no results (immagini gel?). Caterina repeated the digstion but she used two differents type of ligase for the reaction of ligation. At the end of the day she transformed Neb&alpha; with these new products of ligation.   ",
-	"tags" : "Pveg-PliaG-PlepA-plasmidPxil-plasmidPspac"
+	"tags" : "PchBA-BMST1-araC-pBAD"
 }