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Team:UNITN-Trento/Notebook/Labposts/06/66
From 2013.igem.org
(Difference between revisions)
(Created page with "{ "date" : "2013-06-10", "author" : "caterina-fabio", "title" : "The Registry 2013 is deflowered!", "content" : "We extracted <b>BBa_K143012</b>, <b>BBa_K823000</b>, <b>BBa_K...") |
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{ | { | ||
| - | "date" : "2013-06- | + | "date" : "2013-06-29", |
| - | "author" : " | + | "author" : "emil", |
| - | "title" : " | + | "title" : "Purification and screening of the GFPs E0840 and E0240", |
| - | "content" : " | + | "content" : "<html> I did the miniprep of the 8 inocula(2 GFP constructs) following the <a href=\"http://ita.promega.com/~/media/Files/Resources/ProtCards/Wizard%20Plus%20SV%20Minipreps%20DNA%20Purification%20System%20Quick%20Protocol.pdf\">vacuum protocol</a> then I have quantified the products with the following results:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>Quantification</th></tr><tr><td>BBa_E0840/1</td><td> 484.6ng/µl</td></tr><tr><td>BBa_E0840/2</td><td> 376ng/µl</td></tr><tr><td>BBa_E0840/3</td><td> 481.8ng/µl</td></tr><tr><td>BBa_E0840/4</td><td> 475ng/µl</td></tr><tr><th>BBa_E0240/1</th><th> 361ng/µl</th></tr><tr><td>BBa_E0240/2</td><td> 454.4ng/µl</td></tr><tr><td>BBa_E0240/3</td><td> 368ng/µl</td></tr><tr><td>BBa_E0240/4</td><td> 365.8ng/µl</td></tr></table></center></html>}}<html>Then I did the screening of the samples E0840/1,E0840/3,E0240/2,E0240/4. I digested 800 ng of each sample with EcoR1-HF and Pst1-HF following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion\">digestion protocol for screening</a>. After 40 minutes I loaded the samples on a gel with the following results:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel order|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>Well</th></tr><tr><td>Ladder 1kb Fermentas</td><td>1</td></tr><tr><td>BBa_E0840/1</td><td>2</td></tr><tr><td>BBa_E0840/3</td><td>3</td></tr><tr><td>BBa_E0240/2</td><td>4</td></tr><tr><td>BBa_E0240/4</td><td>5</td></tr></table></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|image|<html> <img src=\"https://static.igem.org/mediawiki/2013/4/4b/Tn-20130629-Et_GFP.jpg\" width=\"450px\" /></html>}}<html> We can see 2 bands for well: the upper is the backbone(pSB1A2 2079 bp), the lower is presumably the GFP(BBa_E0240 826 bp,BBa_E0840 828 bp)(pefix and suffix escluded ?).</html>", |
| - | "tags" : " | + | "tags" : "E0840-E0240" |
} | } | ||
Latest revision as of 08:00, 3 October 2013
{ "date" : "2013-06-29", "author" : "emil", "title" : "Purification and screening of the GFPs E0840 and E0240", "content" : " I did the miniprep of the 8 inocula(2 GFP constructs) following the vacuum protocol then I have quantified the products with the following results:
Quantification
Then I did the screening of the samples E0840/1,E0840/3,E0240/2,E0240/4. I digested 800 ng of each sample with EcoR1-HF and Pst1-HF following the digestion protocol for screening. After 40 minutes I loaded the samples on a gel with the following results:| Sample | Quantification |
|---|---|
| BBa_E0840/1 | 484.6ng/µl |
| BBa_E0840/2 | 376ng/µl |
| BBa_E0840/3 | 481.8ng/µl |
| BBa_E0840/4 | 475ng/µl |
| BBa_E0240/1 | 361ng/µl |
| BBa_E0240/2 | 454.4ng/µl |
| BBa_E0240/3 | 368ng/µl |
| BBa_E0240/4 | 365.8ng/µl |
Gel order
We can see 2 bands for well: the upper is the backbone(pSB1A2 2079 bp), the lower is presumably the GFP(BBa_E0240 826 bp,BBa_E0840 828 bp)(pefix and suffix escluded ?).",
"tags" : "E0840-E0240"
}
| Sample | Well |
|---|---|
| Ladder 1kb Fermentas | 1 |
| BBa_E0840/1 | 2 |
| BBa_E0840/3 | 3 |
| BBa_E0240/2 | 4 |
| BBa_E0240/4 | 5 |
![](\"https://static.igem.org/mediawiki/2013/4/4b/Tn-20130629-Et_GFP.jpg\")
