Team:UNITN-Trento/Notebook/Labposts/07/09

From 2013.igem.org

(Difference between revisions)
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{
{
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"date" : "2013-07-24",
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"date" : "2013-07-03",
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"author" : "gabriele",
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"author" : "emil",
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"title" : "Inocula",
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"title" : " Inocula of the plate with the product of ligation and retransformation of the ligation products ",
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"content" : "<html>I checked the plates prepared by Caterina <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-23-caterina\">yesterday</a>.<br/><center><table class=\"tn-sp-table\"><tr><th>plate</th><th>#colonies</th><th>#inocula</th></tr><tr><td>ON Ctrl</td><td>0</td><td>0</td></tr><tr><td>ON 1:1</td><td>6</td><td>5</td></tr><tr><td>ON 1:3</td><td>0</td><td>0</td></tr><tr><td>Short Ctrl</td><td>0</td><td>0</td></tr><tr><td>Short 1:1</td><td>1</td><td>1</td></tr><tr><td>Short 1:3</td><td>1</td><td>1</td></tr></table></center><br/>As you might notice, an O/N 1:3 ligation was never performed, the problem is that Caterina wrote the wrong labels on the plates, so we can't link the plates back to the ligations... anyway, we will keep the denomination of the plates from now onward.</html>",
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"content" : "<html> I observed the plates but unfortunately there were some colonies also in the control (perhaps it was due to degradation of ampicillin when it was added to LB agar too hot) so I have decided to re-transform the products of ligation left following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Competent-cells-transformation\"> transformation protocol</a>.These are the results of the plate:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823026+E0840 1:1 |<html> <img src=\"\" /></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823026+E0840 1:3|<html> <img src=\"\" /></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823024+E0840 1:1|<html> <img src=\"\" /></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823024+E0840 1:3|<html> <img src=\"\" /></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823024+E0840 control|<html> <img src=\"\" /></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823026+E0840 control|<html> <img src=\"\" /></html>}}<html>Moreover I did the inocula of the old plate(3 for K823026+E0840 1:1,3 for K823026+E0840 1:3,3 for K823024+E0840 1:1,3 for K823024+E0840 1:3) in 5 mL of LB with Ampicillin(1:1000).</html>",
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"tags" : "SAMsynthetase"
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"tags" : "K832024-K823026-E0840"
}
}

Revision as of 08:23, 3 October 2013

{ "date" : "2013-07-03", "author" : "emil", "title" : " Inocula of the plate with the product of ligation and retransformation of the ligation products ", "content" : " I observed the plates but unfortunately there were some colonies also in the control (perhaps it was due to degradation of ampicillin when it was added to LB agar too hot) so I have decided to re-transform the products of ligation left following the transformation protocol.These are the results of the plate:

plate K823026+E0840 1:1
plate K823026+E0840 1:3
plate K823024+E0840 1:1
plate K823024+E0840 1:3
plate K823024+E0840 control
plate K823026+E0840 control
Moreover I did the inocula of the old plate(3 for K823026+E0840 1:1,3 for K823026+E0840 1:3,3 for K823024+E0840 1:1,3 for K823024+E0840 1:3) in 5 mL of LB with Ampicillin(1:1000).", "tags" : "K832024-K823026-E0840" }

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