From 2013.igem.org
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| | { | | { |
| - | "date" : "2013-07-24", | + | "date" : "2013-07-04", |
| - | "author" : "thomas", | + | "author" : "fabio-viola", |
| - | "title" : "Screening of AraC-pBAD + EFE + Venus", | + | "title" : "BACILLUS SUBTILIS COMES BACK TO LIFE!", |
| - | "content" : "<html>This is the part five of <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-18-thomas-michele\">this experiment</a>. I proceeded with a <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#miniprep\">miniprep purification</a> of the previously prepared inocula and a <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion\">screening digestion</a> in order to verify the construct. <br/><br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification Results|<html><center><TABLE CLASS=\"tn-sp-table\"><tr><th>Sample</th><th>Concentration</th></tr><tr><td>A from ligation 1:1 </td><td>852.6 ng/ul</td></tr><tr><td>B from ligation 1:1 </td><td>1123 ng/ul</td></tr><tr><td>C from ligation 1:4 </td><td>685.8 ng/ul</td></tr><tr><td>D from ligation 1:4</td><td>2128.3 ng/ul</td></tr><tr><td>E from ligation 1:1 </td><td>1760 ng/ul</td></tr><tr><td>A from ligation 1:1 </td><td>852.6 ng/ul</td></tr></TABLE></center></html>}}<html> <br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel Image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/d/d9/Tn-2013_venus_screening.JPG\" style =\"width: 450px\"></center></html>}}<html> As you can see from the gel image, only one sample seems to confirm our fusion protein and will be sent for sequencing. Lane 4: insert 3024 bp, vector 2070 bp. Kapa universal ladder was adopted.</html>", | + | "content" : "<html> today we made glycerol stock tubes from the remaining cultures and from the inocula made yesterday, but before that we collected some bacteria to make other two liquid cultures just to have more glycerol stock tubes to put at -80°</html>", |
| - | "tags" : "EFE-Venus" | + | "tags" : " Bacillus subtilis " |
| | } | | } |
Revision as of 08:24, 3 October 2013
{
"date" : "2013-07-04",
"author" : "fabio-viola",
"title" : "BACILLUS SUBTILIS COMES BACK TO LIFE!",
"content" : " today we made glycerol stock tubes from the remaining cultures and from the inocula made yesterday, but before that we collected some bacteria to make other two liquid cultures just to have more glycerol stock tubes to put at -80°",
"tags" : " Bacillus subtilis "
}
"
