From 2013.igem.org
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| | { | | { |
| - | "date" : "2013-07-02", | + | "date" : "2013-07-19", |
| - | "author" : "emil", | + | "author" : "caterina", |
| - | "title" : "Purification of the digestion products (K823026, K823024, E0840) ligation of the GFP in the 2 backbones and transformation of the ligation products ", | + | "title" : "SAM sythetase and other miniprep", |
| - | "content" : "<html>This morning I added 1µl of DPN1 to the GFP ligation and 1µl of SAP(alkaline phosphatase) to the 2 backbones.After 1h 30 min I have stopped the reaction by putting the reaction tubes at 80 C°.Afterwards I have purified the products following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Promega-PCR-Gel\"> purification protocol</a>.Then I quantified the products with the following results:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification|<html><table> <tr><th>Sample</th><th>Quantification</th></tr><tr><td>BBa_E0840</td><td> 15.8ng/µl</td></tr><tr><td>BBa_K823026</td><td> 37.6ng/µl</td></tr><tr><td>BBa_K823024</td><td> 36.4ng/µl</td></tr></table></html>}}<html>Then I performed the ligation(1:1,1:3,CTRL) of the GFP with the 2 backbone individually and following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Ligation\"> ligation protocol</a>.Finally I transformed 10µl of the ligation protocol in Neb10β and plate them on ampicillin LB agar following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Competent-cells-transformation\"> transformation protocol</a>.</html>", | + | "content" : "<html>Yesterday, I performed inocula from different plates prepared by Gire. After miniprep and screening we had the confirmation that into pSB1C3 there was RFP and not SAM synthetase. So on Monday we will repeat the experiment.<br/><br/>Then I performed the miniprep of some inocula that I prepared the yesterday containing K1065101 and K1065102.</html>", |
| - | "tags" : "E0840-K823026-K823024" | + | "tags" : "SAM-K1065101-K1065102" |
| | } | | } |
Revision as of 08:34, 3 October 2013
{
"date" : "2013-07-19",
"author" : "caterina",
"title" : "SAM sythetase and other miniprep",
"content" : "Yesterday, I performed inocula from different plates prepared by Gire. After miniprep and screening we had the confirmation that into pSB1C3 there was RFP and not SAM synthetase. So on Monday we will repeat the experiment.
Then I performed the miniprep of some inocula that I prepared the yesterday containing K1065101 and K1065102.",
"tags" : "SAM-K1065101-K1065102"
}
"
