Team:UNITN-Trento/Notebook/Labposts/07/63

From 2013.igem.org

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{
{
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"date" : "2013-07-18",
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"date" : "2013-07-30",
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"author" : "thomas-michele",
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"author" : "Michele",
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"title" : "Back to work",
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"title" : "Come on B. give me some great Spores!! Episode 1",
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"content" : "<html>Today, after a long and hard fight with our thesis, we came back to work. In particular we teased the 2012 Registry Distribution and we extracted two new parts: BBa_K537055 (679 bp) and BBa_K537056 (721 bp). These two parts contained two reportes gene, mCherry and Venus. We did this because we wanted to fuse at the C-term of BBa_K1065001 a reporter in order to measure the kinetic of the protein sythesis. A new chapter to add to our team!  </html>",
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"content" : "<html>Today Emil leave for his holiday and so I took his work in my hands. This was the first time that I met Bacillus in my life... I have to be honest: I didn't fall in love with it immediately!! I tried to make it sporulate by following step by step the 2012 Munich Igem Team \"spore germination assay\"(link protocollo). At the end of the day with Paola we tried to observe the spores in the hemocytometer with the microscope. The spores were clearly visible but we were not able to take a picture.    </html>",
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"tags" : "EFE-mCherry-Venus"
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"tags" : "Bacillus-Sporulation"
}
}

Revision as of 08:46, 3 October 2013

{ "date" : "2013-07-30", "author" : "Michele", "title" : "Come on B. give me some great Spores!! Episode 1", "content" : "Today Emil leave for his holiday and so I took his work in my hands. This was the first time that I met Bacillus in my life... I have to be honest: I didn't fall in love with it immediately!! I tried to make it sporulate by following step by step the 2012 Munich Igem Team \"spore germination assay\"(link protocollo). At the end of the day with Paola we tried to observe the spores in the hemocytometer with the microscope. The spores were clearly visible but we were not able to take a picture. ", "tags" : "Bacillus-Sporulation" }

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