Team:UNITN-Trento/Notebook/Labposts/08/03

From 2013.igem.org

(Difference between revisions)
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{
{
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"date" : "2013-08-04",
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"date" : "2013-08-01",
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"author" : "caterina",
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"author" : "gabriele",
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"title" : "27 minipreps, 27 fails",
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"title" : "pLAC to SAMsynthetase - Ep. III",
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"content" : "<html>Today it was an hard day, after 27 miniprep and 27 screening I didn't obtain positive results, so I restarted the digestion, ligation and trasformation of R0010 + K592009 in pSB1C3 and in pSB1A2.</html>",
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"content" : "<html>I miniprepped the eight inocula from yesterday (great yields, the minimum was 206.3 ng/&micro;l) and screened them with EcoRI-HF and PstI-HF. Five inocula (11A-C, 12B-C-D) were positive!!!</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Agarose Gel|<html><table><tr><th>Loading scheme</th></tr><tr><td>11A</td></tr><tr><td>11B</td></tr><tr><td>11C</td></tr><tr><td>11D</td></tr><tr><td><i>Empty</i></td></tr><tr><td>1kb ladder</td></tr><tr><td><i>Empty</i></td></tr><tr><td>12A</td></tr><tr><td>12B</td></tr><tr><td>12C</td></tr><tr><td>12D</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/4/42/Tn-20130801_ggcm_SAMsynthINpSB1C3withPlac_GOTITfuckyeah.jpg\" /></html>}}<html>One sample was sent to be sequenced.</html>",
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"tags" : "light receptor"
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"tags" : "SAMsynthetase-pLac"
}
}

Revision as of 09:02, 3 October 2013

{ "date" : "2013-08-01", "author" : "gabriele", "title" : "pLAC to SAMsynthetase - Ep. III", "content" : "I miniprepped the eight inocula from yesterday (great yields, the minimum was 206.3 ng/µl) and screened them with EcoRI-HF and PstI-HF. Five inocula (11A-C, 12B-C-D) were positive!!!

Agarose Gel
Loading scheme
11A
11B
11C
11D
Empty
1kb ladder
Empty
12A
12B
12C
12D
One sample was sent to be sequenced.", "tags" : "SAMsynthetase-pLac" }

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