From 2013.igem.org
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| | { | | { |
| | "date" : "2013-08-07", | | "date" : "2013-08-07", |
| - | "author" : "emil", | + | "author" : "gabriele-emil", |
| - | "title" : "Test for integration in B. subtilis ", | + | "title" : "M9 Minimal Medium", |
| - | "content" : "<html>In order to demonstrate the integration of pXyl in Thr locus (essential for the biosinthesis of threonine) I have prepared 40 ml of a minimal medium (MNGE) following the LMU munich protocol without the threonine.Then I did an inoculum from the plate obtained from the transformation of 29/7.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|MNGE medium|<html><center><table><tr><th>Compound</th><th>Amount</th></tr><tr><td>10xMN-Medium</td><td>3,68ml</td></tr><tr><td>Sterile water</td><td>33,12ml</td></tr><tr><td>Glucose (20%)</td><td>4ml</td></tr><tr><td>K-Glutamat (40%)</td><td>200µl</td></tr><tr><td>Fe[III]-ammonium-citrate (2,2 mg/ml)</td><td>200µl</td></tr><tr><td>Tryptophan (5 mg/ml)</td><td>400µl</td></tr><tr><td>MgSO4 (1M)</td><td>120µl</td></tr><tr><td>threonine (5 mg/ml)(in this case absent)</td><td>400µl</td></tr></table></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|MN medium 10x 50 ml|<html><center><table><tr><th>Compound</th><th>Amount</th></tr><tr><td>K2HPO4 (x3 H2O)</td><td>6,8 g</td></tr><tr><td>KH2PO4</td><td>3 g</td></tr><tr><td> Na-citrate (x 2 H2O) </td><td>0.5 g</td></tr></table> </center></html>}}", | + | "content" : "<html>Simply (it is not very easy but we succesed) prepared the M9 minimal medium!</html>", |
| - | "tags" : "B.subtilis-PXyl" | + | "tags" : "M9" |
| | } | | } |
Latest revision as of 09:05, 3 October 2013
{
"date" : "2013-08-07",
"author" : "gabriele-emil",
"title" : "M9 Minimal Medium",
"content" : "Simply (it is not very easy but we succesed) prepared the M9 minimal medium!",
"tags" : "M9"
}
"
