Team:UNITN-Trento/Notebook/Labposts/08/30

From 2013.igem.org

(Difference between revisions)
 
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{
{
"date" : "2013-08-13",
"date" : "2013-08-13",
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"author" : "viola",
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"author" : "fabio",
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"title" : "I won!!",
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"title" : " blue light induction",
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"content" : "<html>Today i've had the results of the sequencing of pXyl+EFE+B0015 and of pSpac+EFE+B0015 and...they were finally completely right!!!! so i transformed the right DNA in order to amplify a lot of DNA to keep on with the bacillus transformations. </html>",
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"content" : "<html> today I made some dilutions 1:100 from the inocula, to have 3 cultures (200 ml) for the experiment: one will be induced with blue light, the other two are control sample, the fors exposed to standard light, the last one will stay “alone in the dark”. I started the experiment when the dilution cultures reached 0.7 OD. After 4 ours from the beginning of the induction I took 10 ml from the samples and obtained pellet to see blue pigment production: no difference. The experiment will go on over the night, tomorrow we will hopefully be able to see some difference. Meanwhile I made some inocula from the same plate, in order to repeat the experiment with less volume cultures.</html>",
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"tags" : "pXyl-EFE-B0015-pSpac"
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"tags" : " BlueLight_amilCP"
}
}

Latest revision as of 09:10, 3 October 2013

{ "date" : "2013-08-13", "author" : "fabio", "title" : " blue light induction", "content" : " today I made some dilutions 1:100 from the inocula, to have 3 cultures (200 ml) for the experiment: one will be induced with blue light, the other two are control sample, the fors exposed to standard light, the last one will stay “alone in the dark”. I started the experiment when the dilution cultures reached 0.7 OD. After 4 ours from the beginning of the induction I took 10 ml from the samples and obtained pellet to see blue pigment production: no difference. The experiment will go on over the night, tomorrow we will hopefully be able to see some difference. Meanwhile I made some inocula from the same plate, in order to repeat the experiment with less volume cultures.", "tags" : " BlueLight_amilCP" }