Team:UNITN-Trento/Notebook/Labposts/08/39

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{
{
"date" : "2013-08-15",
"date" : "2013-08-15",
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"author" : "emil",
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"author" : "viola-fabio",
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"title" : "Emil's(re)begins",
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"title" : "Verifying the triple ligation",
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"content" : "<html>I screened 5&micro;l of the two PCR that I started the day before(5 of K823025 and 2 of E0840, These are the    results:</html>      {{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel image|<html> <img src=''/></html>}}<html>Unfortunately there isn't the ladder I loaded only the pure dye, otherwise can reasonably consider the PCR correct, after this I purified 3 backbone and the 2 GFP following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#Promega-PCR-Gel'>purification protocol</a> with the following results:</html>
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"content" : "<html>In order to verify the triple ligation performed <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-08-14-viola-thomas\">the day before yesterday</a> Fabio did some inocula from the colonies of the 1:2 condition plate. </html>",
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      {{:Team:UNITN-Trento/Templates/Styles/Spoiler|results|<html>
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"tags" : "pFixK-cI-pLambda-EFE"
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        <center>
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            <table>
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                <td>
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                  <th>Sample</th>
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                  <th>Quantities</th>
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                </td>
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                <td>
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                    <tr>GFP 1</tr>
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                    <tr>70.5 ng/6micro;l</tr>
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                </td>
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                <td>
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                    <tr>GFP 2</tr>
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                    <tr>52 ng/6micro;l</tr>
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                </td>
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                <td>
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                    <tr>PXyl 1</tr>
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                    <tr>110.4 ng/6micro;l</tr>
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                </td>
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                <td>
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                    <tr>Pxyl 2</tr>
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                    <tr>77.7 ng/6micro;l</tr>
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                </td>
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                <td>
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                    <tr>Pxyl 3</tr>
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                    <tr>137 ng/6micro;l</tr>
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                </td>
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            </table>
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          </center>
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        </html>}}
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    <html>After that I did a digestion overnight of 3 &micro;l of the GFP1 and the PXyl1 following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion'> digestion protocol</a>.</html>",
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"tags" : "PXyl-GFP"
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}
}

Latest revision as of 09:13, 3 October 2013

{ "date" : "2013-08-15", "author" : "viola-fabio", "title" : "Verifying the triple ligation", "content" : "In order to verify the triple ligation performed the day before yesterday Fabio did some inocula from the colonies of the 1:2 condition plate. ", "tags" : "pFixK-cI-pLambda-EFE" }

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