From 2013.igem.org
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| | { | | { |
| - | "date" : "2013-08-17", | + | "date":"2013-08-16", |
| - | "author" : "emil", | + | "author":"viola-caterina", |
| - | "title" : "To be continued 2 ", | + | "title":"<html>A night in da (C)LAB</html>", |
| - | "content" : "<html> I verified that the transformation has succeded only in one case (1:1) with 4 colonies so I performed the miniprep following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#miniprep'> protocol </a>, after that I tested the construct by digestion with Sca1 following the Thermo scientific protocol for Sca1 digestion with the following result:</html> {{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel image|<html> <img src='https://static.igem.org/mediawiki/2013/b/b8/Tn-2013_Foto_%288%29.JPG' width='400px;'></html>}}<html> as you can see in the second lane ther is a single bright band at 6000 bp which correspond to the final construct, this construct were transformed in 400 µl <i>Bacillus</i> cells growth at O.D. 1.1 in Minimal medium following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#subtilis-transformation'> transformation protocol </a>. These cells were plated on Spectinomicin added Agar.</html>",
| + | "content":"<html>We passed a really beautiful night in the lab starting a cloning short protocol at 19:30 and plating the cells at 5:00 am.... we are really mad!!!!! </html>", |
| - | "tags" : "pXyl-GFP" | + | "tags":"EFE" |
| | } | | } |
Latest revision as of 09:15, 3 October 2013
{
"date":"2013-08-16",
"author":"viola-caterina",
"title":"A night in da (C)LAB",
"content":"We passed a really beautiful night in the lab starting a cloning short protocol at 19:30 and plating the cells at 5:00 am.... we are really mad!!!!! ",
"tags":"EFE"
}
"
