Team:UNITN-Trento/Notebook/Labposts/08/47
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(Created page with " { "date" : "2013-08-20", "author" : "Aor Bruno", "title" : "<html>Mutagenesis, the revenge</html>", "content" : "<html>After the creation of K1065300 (without RBS) I doing t...") |
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{ | { | ||
- | "date" : "2013-08- | + | "date" : "2013-08-18", |
- | "author" : " | + | "author" : "thomas", |
- | "title" : "<html> | + | "title" : "<html>Preparing sequencing samples of AraC-pBAD EFE</html>", |
- | "content" : "<html> | + | "content" : "<html>This morning I got to prepares samples of AraC-pBAD EFE to send them for sequencing. We decided to sequence both samples with Vf and Vr primers. So knowing the preps concentrations, I prepared the mix as follow:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification results|<html><table><tr><th></th><th>Volume added to sample 1:1 (µl)</th><th>Volume added to sample 1:2 (µl)</th></tr><tr><th>Template DNA</th><td>5.5</td><td>6.5</td></tr><tr><th>Primer</th><td>2</td><td>2</td></tr><tr><th>Water</th><td>up to 15</td><td>up to 15</td></tr></table></html>}}<html> The samples were then putted in a falcon and send with a courier. </html>", |
- | "tags" : " | + | "tags" : "aracpbad-efe" |
} | } |
Latest revision as of 09:16, 3 October 2013
{ "date" : "2013-08-18", "author" : "thomas", "title" : "Preparing sequencing samples of AraC-pBAD EFE", "content" : "This morning I got to prepares samples of AraC-pBAD EFE to send them for sequencing. We decided to sequence both samples with Vf and Vr primers. So knowing the preps concentrations, I prepared the mix as follow:
Quantification results
The samples were then putted in a falcon and send with a courier. ",
"tags" : "aracpbad-efe"
}
Volume added to sample 1:1 (µl) | Volume added to sample 1:2 (µl) | |
---|---|---|
Template DNA | 5.5 | 6.5 |
Primer | 2 | 2 |
Water | up to 15 | up to 15 |