Team:UNITN-Trento/Notebook/Labposts/08/57

From 2013.igem.org

(Difference between revisions)

Latest revision as of 09:18, 3 October 2013

{ "date" : "2013-08-31", "author" : "thomas", "title" : "Sequencing and reamplification of AraC-pBAD EFE", "content" : "This morning I had to reamplify the construnct AraC-pBAD EFE in order to have a stock of it. So I starting from the inocula previously prepared, I did miniprep purification and quantification of six samples obtaining the following concentrations.

Quantification results

Sample	Concentration (ng/µl)
1:1 sample 1	383
1:1 sample 2	174.8
1:1 sample 3	182.4
1:2 sample 1	173.9
1:2 sample 2	154.3
1:2 sample 3	188

The construct was successfully reamplified!", "tags" : "aracpbad-efe" }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-08-07",
+	"date" : "2013-08-31",
-	"author" : "gabriele-emil",
+	"author" : "thomas",
-	"title" : "M9 Minimal Medium",
+	"title" : "<html>Sequencing and reamplification of AraC-pBAD EFE</html>",
-	"content" : "<html>Simply (it is not very easy but we succesed) prepared the M9 minimal medium!</html>",
+	"content" : "<html>This morning I had to reamplify the construnct AraC-pBAD EFE in order to have a stock of it. So I starting from the inocula previously prepared, I did miniprep purification and quantification of six samples obtaining the following concentrations.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification results|<html><table><tr><th>Sample</th><th>Concentration (ng/&micro;l)</th></tr><tr><td>1:1 sample 1</td><td>383</td></tr><tr><td>1:1 sample 2</td><td>174.8</td></tr><tr><td>1:1 sample 3</td><td>182.4</td></tr><tr><td>1:2 sample 1</td><td>173.9</td></tr><tr><td>1:2 sample 2</td><td>154.3</td></tr><tr><td>1:2 sample 3</td><td>188</td></tr></table></html>}}<html> The construct was successfully reamplified!</html>",
-	"tags" : "M9"
+	"tags" : "aracpbad-efe"
 }