Team:UNITN-Trento/Notebook/Labposts/08/02

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(Created page with "{ "date" : "2013-08-02", "author" : "Michele", "title" : "Doing a mess", "content" : "<html>So, I didn't believe Cristina when in July she told us: \"In August we're going to...")
 
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{
{
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"date" : "2013-08-02",
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"date" : "2013-08-01",
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"author" : "Michele",
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"author" : "thomas",
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"title" : "Doing a mess",
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"title" : "Overnight cultures",
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"content" : "<html>So, I didn't believe Cristina when in July she told us: \"In August we're going to work twice than this\" but I had better trust her. Today I did two TLC on four different samples to detect MeSA presence. </html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|TLC Image|<html><center><img src=\"https://2013.igem.org/File:Tn-2013_TLC_Fail.JPG\" width=\"450px\" /></center></html>}}<html> As you can see on the image of one of the two TLC only the control (MeSA pure diluited 1:1 in CH2Cl2) ran. These was the third TLC that failed then we decided to avoid more TLC tests. During the day I checked also the growth of Bacillus (link previous post sporulation). In the crazy afternoon after an hard fight with the order in the Lab I performed a screening on GFP (Bba_E0840) to verify if our stock was correct.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel Image|<html><center><img src=\"https://2013.igem.org/File:Tn-2013_Gel_screening_GFP_BBa_E0840.jpg\" width=\"450px\" /></center></html>}}<html>As you can is present the band at 878 bp so the screening gave positive results. During the digestion and the electrophoretic run I also did a transforamtion of Neb10&beta; with EFE in pSB1C3. </html>",
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"content" : "<html>Starting with the plates I did <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-31-thomas\">yesterday</a>, I prepared the following overnight cultures. I did that also with some colonies transformed with pXyl (BBa_K823024) + GFP (BBa_E0840) that Fabio gave me in order to amplify his construct.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Overnight cultures prepared|<html><center><table><tr><th>Plate</th><th>N° of colonies taken</th></tr><tr><td>pXyl + GFP </td><td>3</td></tr><tr><td>pSpac + GFP ligation 1:1 </td><td>2</td></tr><tr><td>pSpac + GFP ligation 1:4</td><td>3</td></tr></table></center></html>}}<html> </html>",
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"tags" : "GFP-EFE-TLC"
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"tags" : "pSpac-pXyl-GFP"
}
}

Latest revision as of 09:43, 3 October 2013

{ "date" : "2013-08-01", "author" : "thomas", "title" : "Overnight cultures", "content" : "Starting with the plates I did yesterday, I prepared the following overnight cultures. I did that also with some colonies transformed with pXyl (BBa_K823024) + GFP (BBa_E0840) that Fabio gave me in order to amplify his construct.

Overnight cultures prepared
PlateN° of colonies taken
pXyl + GFP 3
pSpac + GFP ligation 1:1 2
pSpac + GFP ligation 1:43
", "tags" : "pSpac-pXyl-GFP" }

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