Team:UNITN-Trento/Notebook/Labposts/07/09

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(Difference between revisions)

Latest revision as of 10:34, 3 October 2013

{"date" : "2013-07-03","author" : "gabriele","title" : "An easy day...","content" : "First of all I counted the colonies from yesterday:

plate	#colonies
Ctrl	4
0.5:1	2
0.5:2	0

Then, I tried again to linearize pSB1C3 (this time, starting from the circular), but I failed.

Gel

Loading scheme
1kb ladder	G1	G2	G3

$\"Gel\"$

","tags" : "SAMsynthetase-pSB1C3"}

@@ Line 1: / Line 1: @@
-{
+{"date" : "2013-07-03","author" : "gabriele","title" : "An easy day...","content" : "<html>First of all I counted the colonies from <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-02-gabriele\">yesterday</a>:<br/><br/><center><table class=\"tn-sp-table\"><tr><th>plate</th><th>#colonies</th></tr><tr><td>Ctrl</td><td>4</td></tr><tr><td>0.5:1</td><td>2</td></tr><tr><td>0.5:2</td><td>0</td></tr></table></center><br/><hr><br/>Then, I tried again to linearize pSB1C3 (this time, starting from the circular), but I failed.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel|<html><center><table class=\"tn-sp-table\"><tr><th colspan=\"4\">Loading scheme</th></tr><tr><td>1kb ladder</td><td>G1</td><td>G2</td><td>G3</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/d/d3/Tn-20130703-GGCM_linearizationpSB1C3_FAILED.jpg\" alt=\"Gel\" width=\"450px\"></center></html>}}<html></html>","tags" : "SAMsynthetase-pSB1C3"}
-	"date" : "2013-07-24",
-	"author" : "gabriele",
-	"title" : "Inocula",
-	"content" : "<html>I checked the plates prepared by Caterina <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-23-caterina\">yesterday</a>.<br/><center><table class=\"tn-sp-table\"><tr><th>plate</th><th>#colonies</th><th>#inocula</th></tr><tr><td>ON Ctrl</td><td>0</td><td>0</td></tr><tr><td>ON 1:1</td><td>6</td><td>5</td></tr><tr><td>ON 1:3</td><td>0</td><td>0</td></tr><tr><td>Short Ctrl</td><td>0</td><td>0</td></tr><tr><td>Short 1:1</td><td>1</td><td>1</td></tr><tr><td>Short 1:3</td><td>1</td><td>1</td></tr></table></center><br/>As you might notice, an O/N 1:3 ligation was never performed, the problem is that Caterina wrote the wrong labels on the plates, so we can't link the plates back to the ligations... anyway, we will keep the denomination of the plates from now onward.</html>",
-	"tags" : "SAMsynthetase"
-}