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Team:UNITN-Trento/Notebook/Labposts/07/10
From 2013.igem.org
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| - | { | + | {"date" : "2013-07-03","author" : "fabio-viola","title" : "BACILLUS SUBTILIS COMES BACK TO LIFE!","content" : "<html> HORROR!! We lost kind of the whole bacillus that we had, during the night, cause the liquid cultures were disrupted in the shaker at 26!! Luckily we have a survivor, the liquid culture at 37, and all the plates. <br>From the survivor we made further liquid cultures ( at 26°, 1:50 and 1:100; at 37° 1:50 and 1:100) <br>As soon as they became very cloudy we went along with the glycerol stock preparation and put our bacillus at -80°. The one that weren’t cloudy have been kept in the shaker all night long. <br>In the afternoon we inoculated some colonies from the plates in 5 ml of nutrient broth +starch.</html>","tags" : " Bacillus subtilis"} |
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Latest revision as of 10:34, 3 October 2013
{"date" : "2013-07-03","author" : "fabio-viola","title" : "BACILLUS SUBTILIS COMES BACK TO LIFE!","content" : " HORROR!! We lost kind of the whole bacillus that we had, during the night, cause the liquid cultures were disrupted in the shaker at 26!! Luckily we have a survivor, the liquid culture at 37, and all the plates.
From the survivor we made further liquid cultures ( at 26°, 1:50 and 1:100; at 37° 1:50 and 1:100)
As soon as they became very cloudy we went along with the glycerol stock preparation and put our bacillus at -80°. The one that weren’t cloudy have been kept in the shaker all night long.
In the afternoon we inoculated some colonies from the plates in 5 ml of nutrient broth +starch.","tags" : " Bacillus subtilis"}
