Team:UNITN-Trento/Notebook/Labposts/07/22

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(Difference between revisions)

Latest revision as of 10:37, 3 October 2013

{"date" : "2013-07-09","author" : "Bruno","title" : "Ripenator v1.0","content" : "I set up a system to ripening or block ripening a set of green (immature) bananas.
The idea is to grow into a beaker our strain of E. coli that produces ethylene, and connect it to a laboratory dryer with bananas. In this way, E. coli (be stirred and 37 ° C), producing Ethylene, should increase the maturation of the banana in the dryer connected.
In addition, we have included in another beaker Mesa pure with LB, which should slow down the ripening at high concentration and should promote the ripening at low concentrations.","tags" : "Ripenator-Mesa-Ethylene"}

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+{"date" : "2013-07-09","author" : "Bruno","title" : "Ripenator v1.0","content" : "<html>I set up a system to ripening or block ripening a set of green (immature) bananas. <br>The idea is to grow into a beaker our strain of E. coli that produces ethylene, and connect it to a laboratory dryer with bananas. In this way, E. coli (be stirred and 37 ° C), producing Ethylene, should increase the maturation of the banana in the dryer connected. <br>In addition, we have included in another beaker Mesa pure with LB, which should slow down the ripening at high concentration and should promote the ripening at low concentrations.</html>","tags" : "Ripenator-Mesa-Ethylene"}
-	"date" : "2013-07-25",
-	"author" : "fabio",
-	"title" : " the blue ligation: the never ending story !",
-	"content" : "<html> today I miniprepped and screened (on a medium and a large size gel) the 6 ligation inocula! <br></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel|<html><center><img src=\"https://static.igem.org/mediawiki/2013/c/ce/Tn-2013Eee.jpg\" width=\"450px\" /></center></html>}}<html><br>As we can see from the gel the ligation seems not to be occurred!! I forgot to run the plasmid (016) alone to see a difference with the ligations though!! However I need to digest k592016 again to repeat another ligation with the blu promoter!!this time I digested 3 ug of the A sample from the first set!!</html>",
-	"tags" : " YF1_FixJ - FixK2"
-}