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Team:UNITN-Trento/Notebook/Labposts/07/24
From 2013.igem.org
(Created page with "{ "date" : "2013-07-28", "author" : "fabio", "title" : " the blue ligation: the never ending story !", "content" : "<html> today I started with the fourth ligation and then ...") |
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| - | { | + | {"date" : "2013-07-10","author" : "fabio","title" : " ripenator v1.0 ","content" : "<html> this morning, Bruno is not available, so I will take care of the ripenator. I have to replace all the cultures with new LB and new bacteria : I’m gonna put 300 ml of LB in each beutas, then connect immediately a beuta with LB and 3ml of not-induced bacteria to the control banana!! After that, I will dissolve 78 ul of MESA in the second beuta and connect to the blocked banana! As far as the ethylene sample is concerned, I have to put 3 ml of bacteria in the last beuta, wait for the OD lecture to reach the value of 0,5 and then induce the culture with Arabinose to produce Ethylene! Finally I can connect it with the mature banana. </html>","tags" : " ripenator-mesa-ethylene "} |
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Latest revision as of 10:37, 3 October 2013
{"date" : "2013-07-10","author" : "fabio","title" : " ripenator v1.0 ","content" : " this morning, Bruno is not available, so I will take care of the ripenator. I have to replace all the cultures with new LB and new bacteria : I’m gonna put 300 ml of LB in each beutas, then connect immediately a beuta with LB and 3ml of not-induced bacteria to the control banana!! After that, I will dissolve 78 ul of MESA in the second beuta and connect to the blocked banana! As far as the ethylene sample is concerned, I have to put 3 ml of bacteria in the last beuta, wait for the OD lecture to reach the value of 0,5 and then induce the culture with Arabinose to produce Ethylene! Finally I can connect it with the mature banana. ","tags" : " ripenator-mesa-ethylene "}
