Team:UNITN-Trento/Notebook/Labposts/07/38

From 2013.igem.org

(Difference between revisions)

Latest revision as of 10:41, 3 October 2013

{"date" : "2013-07-18","author" : "emil","title" : "The incompetent Bacillus subtilis saga medium episode ","content" : "I have prepared a minimal medium for bacillus following the Cambridge Igem 2012 protocol and I did an inocula of bacillus from a glicerol stock 50% for the following day.Moreover I tried to grow some competent NEB10β following the competent cell protocol .","tags" : "Bacillus_subtilis"}

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+{"date" : "2013-07-18","author" : "emil","title" : "The incompetent Bacillus subtilis saga medium episode ","content" : "<html>I have prepared a minimal medium for bacillus following the Cambridge Igem 2012 protocol and I did an inocula of bacillus from a glicerol stock 50% for the following day.Moreover I tried to grow some competent NEB10&beta; following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Competent-cells-prep\"> competent cell protocol </a>.</html>","tags" : "Bacillus_subtilis"}
-	"date" : "2013-07-03",
-	"author" : "fabio-viola",
-	"title" : "BACILLUS SUBTILIS COMES BACK TO LIFE!",
-	"content" : "<html> HORROR!! We lost kind of the whole bacillus that we had, during the night, cause the liquid cultures were disrupted in the shaker at 26!! Luckily we have a survivor, the liquid culture at 37, and all the plates. <br>From the survivor we made further liquid cultures ( at 26°, 1:50 and 1:100; at 37° 1:50 and 1:100) <br>As soon as they became very cloudy we went along with the glycerol stock preparation and put our bacillus at -80°. The one that weren’t cloudy have been kept in the shaker all night long. <br>In the afternoon we inoculated some colonies from the plates in 5 ml of nutrient broth +starch.</html>",
-	"tags" : " Bacillus subtilis"
-}