From 2013.igem.org
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| - | { | + | {"date" : "2013-07-22","author" : "caterina","title" : "Titolo","content" : "<html>I performed the digestion o/n of SAM synthetase and R0010 in pSB1C3.</html>","tags" : "SAMsynthetase"} |
| - | "date" : "2013-07-23",
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| - | "author" : "emil",
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| - | "title" : "The incompetent Bacillus subtilis saga: The LB transformation and amplification of pSpac +GFP",
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| - | "content" : "<html>I have transformed 3 groups of cells with 500 ng of pSpac+pLac+RFP,I grew Bacillus in LB and when it reached OD of 1.1 I added the DNA to 400µl of cells,I let grow for 1 hour and then I plated the cell on Kanamicin plates.Afterwards I transformed Neb10β with 100 ng of K823026+E0840 following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Competent-cells-transformation\">transformation protocol</a>.</html>",
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| - | "tags" : "Bacillus subtilis-pSpac+GFP"
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| - | } | + | |
Latest revision as of 10:43, 3 October 2013
{"date" : "2013-07-22","author" : "caterina","title" : "Titolo","content" : "I performed the digestion o/n of SAM synthetase and R0010 in pSB1C3.","tags" : "SAMsynthetase"}
"
