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Team:UNITN-Trento/Notebook/Labposts/07/56
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(Created page with "{ "date" : "2013-07-12", "author" : "emil", "title" : "Competent cells", "content" : "<html>I did some competent cells according to the <a href=\"https://2013.igem.org/Team:UN...") |
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| - | { | + | {"date" : "2013-07-24","author" : "fabio-bruno","title" : " the blue ligation: the never ending story !","content" : "<html>Today I inoculated 6 colonies of the plated ligation ( 3 from the 1:1 plate and 2 from the 1:3 plate) <br>I also made the Onetaq Pcr for all the light parts ( blue device,the b sample from the first set; red device, from the third set; and red promoter, from the third set). <br>After that I screened them all and found out that only the blue part was confirmed: up to now we only have the blue parts confirmed!! Red parts , it seems to me that you’re in hot waters !! <br></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Immagine gel|<html><center><img src=\"https://static.igem.org/mediawiki/2013/7/7f/Tn-2013_Daa.jpg\" width=\"450px\" /></center></html>}}<html></html>","tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"} |
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Latest revision as of 10:45, 3 October 2013
{"date" : "2013-07-24","author" : "fabio-bruno","title" : " the blue ligation: the never ending story !","content" : "Today I inoculated 6 colonies of the plated ligation ( 3 from the 1:1 plate and 2 from the 1:3 plate)
I also made the Onetaq Pcr for all the light parts ( blue device,the b sample from the first set; red device, from the third set; and red promoter, from the third set).
After that I screened them all and found out that only the blue part was confirmed: up to now we only have the blue parts confirmed!! Red parts , it seems to me that you’re in hot waters !!
![](\"https://static.igem.org/mediawiki/2013/7/7f/Tn-2013_Daa.jpg\")
