Team:UNITN-Trento/Notebook/Labposts/07/57

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(Difference between revisions)

Latest revision as of 10:45, 3 October 2013

{"date" : "2013-07-24","author" : "gabriele","title" : "Inocula","content" : "I checked the plates prepared by Caterina yesterday.

plate	#colonies	#inocula
ON Ctrl	0	0
ON 1:1	6	5
ON 1:3	0	0
Short Ctrl	0	0
Short 1:1	1	1
Short 1:3	1	1

As you might notice, an O/N 1:3 ligation was never performed, the problem is that Caterina wrote the wrong labels on the plates, so we can't link the plates back to the ligations... anyway, we will keep the denomination of the plates from now onward.","tags" : "SAMsynthetase"}

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+{"date" : "2013-07-24","author" : "gabriele","title" : "Inocula","content" : "<html>I checked the plates prepared by Caterina <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-23-caterina\">yesterday</a>.<br/><center><table class=\"tn-sp-table\"><tr><th>plate</th><th>#colonies</th><th>#inocula</th></tr><tr><td>ON Ctrl</td><td>0</td><td>0</td></tr><tr><td>ON 1:1</td><td>6</td><td>5</td></tr><tr><td>ON 1:3</td><td>0</td><td>0</td></tr><tr><td>Short Ctrl</td><td>0</td><td>0</td></tr><tr><td>Short 1:1</td><td>1</td><td>1</td></tr><tr><td>Short 1:3</td><td>1</td><td>1</td></tr></table></center><br/>As you might notice, an O/N 1:3 ligation was never performed, the problem is that Caterina wrote the wrong labels on the plates, so we can't link the plates back to the ligations... anyway, we will keep the denomination of the plates from now onward.</html>","tags" : "SAMsynthetase"}
-	"date" : "2013-07-27",
-	"author" : "thomas",
-	"title" : "AraCpBAD + EFE + Venus transformation",
-	"content" : "<html>Once I confirmed the construct through an electrophoresis analisis, I decided to transorm the sample C into NEB10b cells. I plated then 100 ul of cells and putted the plate in static incubation at 37&deg;C overnight.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Plate Image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/5/53/Tn-2013_efe%2Bvenus_plate.JPG\" style =\"width: 450px\"></center></html>}}<html> As you can see from the picture, the colonies grown. One of them was then picked up and inoculated overnight.</html>",
-	"tags" : "EFE-Venus"
-}