Team:UNITN-Trento/Notebook/Labposts/07/60

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(Difference between revisions)

Latest revision as of 10:46, 3 October 2013

{"date" : "2013-07-25","author" : "fabio","title" : " the blue ligation: the never ending story !","content" : " today I miniprepped and screened (on a medium and a large size gel) the 6 ligation inocula!

gel

As we can see from the gel the ligation seems not to be occurred!! I forgot to run the plasmid (016) alone to see a difference with the ligations though!! However I need to digest k592016 again to repeat another ligation with the blu promoter!!this time I digested 3 ug of the A sample from the first set!!","tags" : " YF1_FixJ - FixK2"}

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+{"date" : "2013-07-25","author" : "fabio","title" : " the blue ligation: the never ending story !","content" : "<html> today I miniprepped and screened (on a medium and a large size gel) the 6 ligation inocula! <br></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel|<html><center><img src=\"https://static.igem.org/mediawiki/2013/c/ce/Tn-2013Eee.jpg\" width=\"450px\" /></center></html>}}<html><br>As we can see from the gel the ligation seems not to be occurred!! I forgot to run the plasmid (016) alone to see a difference with the ligations though!! However I need to digest k592016 again to repeat another ligation with the blu promoter!!this time I digested 3 ug of the A sample from the first set!!</html>","tags" : " YF1_FixJ - FixK2"}
-	"date" : "2013-07-15",
-	"author" : "thomas",
-	"title" : "Ethylene production kinetic assay (third and last trial)",
-	"content" : "<html>Since I was not satisfied with the curve obtained during the <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-08-thomas\">last experiment</a>, I decided to redo the experiment, this time starting from a culture with O.D.600 = 0,8 and registering a measurment every 45 min.  <br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Plot|<html><center><img src=\"https://static.igem.org/mediawiki/2013/0/00/Tn-2013_kinetic_EFE_plot-2.png\" style =\"width: 450px\"></center></html>}}<html>Interestingly, inducing with arabinose when the sample has reached OD600 0.8 causes an increase of more than two fold in the production of ethylene.</html>",
-	"tags" : "EFE"
-}