Team:UNITN-Trento/Notebook/Labposts/07/61

From 2013.igem.org

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(Created page with "{ "date" : "2013-07-16", "author" : "gabriele", "title" : "A short day", "content" : "<html>I added 1µl of DpnI to G1_EX-SAMsynth-SP O/N digestion and 1µl of SAP ...")
 
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{"date" : "2013-07-26","author" : "viola","title" : "<html>I'm near the solution...</html>","content" : "<html>Poor bacillus!!! Fried in a super basic pH...the media that we used were too basic! so tonight i tried to bring to pH 7 the Groningen's media and tomorrow wi we'll see the truth... meanwhile i prepared the samples for the second sequencing of pspac and pxyl vectors with EFE... i hope that this time there will be something good!!!!  </html>","tags" : "Bacillus"}
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"date" : "2013-07-16",
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"author" : "gabriele",
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"title" : "A short day",
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"content" : "<html>I added 1&micro;l of DpnI to G1_EX-SAMsynth-SP O/N digestion and 1&micro;l of SAP to G2A_linear-pSB1C3 O/N digestion, and then incubated both samples at 37&deg;C for 1.5h. Finally, I stocked both samples at -20&deg;C because I would like to ligate them together with the short digestion that I am going to perform tomorrow.</html>",
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"tags" : "SAMsynthetase"
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}
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Latest revision as of 10:46, 3 October 2013

{"date" : "2013-07-26","author" : "viola","title" : "I'm near the solution...","content" : "Poor bacillus!!! Fried in a super basic pH...the media that we used were too basic! so tonight i tried to bring to pH 7 the Groningen's media and tomorrow wi we'll see the truth... meanwhile i prepared the samples for the second sequencing of pspac and pxyl vectors with EFE... i hope that this time there will be something good!!!! ","tags" : "Bacillus"}