Team:UNITN-Trento/Notebook/Labposts/07/64

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(Difference between revisions)

Latest revision as of 10:47, 3 October 2013

{"date" : "2013-07-27","author" : "thomas","title" : "AraCpBAD + EFE + Venus transformation","content" : "Once I confirmed the construct through an electrophoresis analisis, I decided to transorm the sample C into NEB10b cells. I plated then 100 ul of cells and putted the plate in static incubation at 37°C overnight.

Plate Image

As you can see from the picture, the colonies grown. One of them was then picked up and inoculated overnight.","tags" : "EFE-Venus"}

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+{"date" : "2013-07-27","author" : "thomas","title" : "AraCpBAD + EFE + Venus transformation","content" : "<html>Once I confirmed the construct through an electrophoresis analisis, I decided to transorm the sample C into NEB10b cells. I plated then 100 ul of cells and putted the plate in static incubation at 37&deg;C overnight.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Plate Image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/5/53/Tn-2013_efe%2Bvenus_plate.JPG\" style =\"width: 450px\"></center></html>}}<html> As you can see from the picture, the colonies grown. One of them was then picked up and inoculated overnight.</html>","tags" : "EFE-Venus"}
-	"date" : "2013-07-19",
-	"author" : "caterina",
-	"title" : "SAM sythetase and other miniprep",
-	"content" : "<html>Yesterday, I performed inocula from different plates prepared by Gire. After miniprep and screening we had the confirmation that into pSB1C3 there was RFP and not SAM synthetase. So on Monday we will repeat the experiment.<br/><br/>Then I performed the miniprep of some inocula that I prepared the yesterday containing K1065101 and K1065102.</html>",
-	"tags" : "SAM-K1065101-K1065102"
-}