Team:UNITN-Trento/Notebook/Labposts/07/75

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(Difference between revisions)

Latest revision as of 10:49, 3 October 2013

{"date" : "2013-07-31","author" : "fabio","title" : " the blue ligation: the never ending story !","content" : "Today massive miniprepping y’all!! This is a wonderful day because one of my miniprep seems toOk!! Sample number 3 from the 3rd ligation doesn’t show the band that correspond to 016 not ligated to the 006 promoter! Tomorrow I’ll need to verify by onetaq pcr.

Immagine gel

","tags" : "YF1_FixJ-FixK2"}

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+{"date" : "2013-07-31","author" : "fabio","title" : " the blue ligation: the never ending story !","content" : "<html>Today massive miniprepping y’all!! This is a wonderful day because one of my miniprep seems toOk!! Sample number 3 from the 3rd ligation doesn’t show the band that correspond to  016 not ligated to the 006 promoter! Tomorrow I’ll need to verify by onetaq pcr.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Immagine gel|<html><center><img src=\"https://static.igem.org/mediawiki/2013/3/3a/Tn-2013_Fff.jpg\" width=\"450px\" /></center></html>}}<html></html>","tags" : "YF1_FixJ-FixK2"}
-"date":"2013-07-02",
-"author":"viola",
-"title":"<html>EFE+B0015</html>",
-"content":"<html>today i digested B015 in pSB1C3 with the enzymes E and X and EFE in pUC57 with E and S. I started a short cloning protocol with this ligation quantities: <table> <tr> <th>LIGATION</th> <th>CTRL</th> <th>1:1</th> <th>1:2</th> <th>1:4</th> </tr> <tr> <th>BUFFER T4</th> <td>2 µl</td> <td>2 µl</td> <td>2 µl</td> <td>2 µl</td> </tr> <tr> <th>PLASMID (B0015)</th> <td>2.5 µl</td> <td>2.5 µl</td> <td>2.5 µl</td> <td>2.5 µl</td> </tr> <tr> <th>INSEERT (EFE) </th> <td>0 µl</td> <td>1.28 µl</td> <td>2.56 µl</td> <td>5.12 µl</td> </tr> <tr> <th>LIGASE T4 </th> <td>1 µl</td> <td>1 µl</td> <td>1 µl</td> <td>1 µl</td> </tr> <tr> <th>H2O </th> <td>14.5 µl</td> <td>13.2 µl</td> <td>11.9 µl</td> <td>10.6 µl</td> </tr> </table> </html>",
-"tags":"EFE"
-}