Team:EPF Lausanne/Calendar/8 September 2013

From 2013.igem.org

(Difference between revisions)
(Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of MMP9 insert and GelE insert''' <BR> I did the PCR of the MMP9 and the gelE insert which worked. I then purified it which gave...")
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Sensing <BR>
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<font size = "4"> Sensing </font> <BR>
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'''PCR of MMP9 insert and GelE insert''' <BR>
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''PCR of MMP9 insert and GelE insert'' <BR>
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I did the PCR of the MMP9 and the gelE insert which worked. I then purified it which gave me concentrations around 100ng/ul.  
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-I did the PCR of the MMP9 and the gelE insert which worked. I then purified it which gave me concentrations around 100ng/ul.  
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'''DpnI digest of MMP2 insert and MMP2 Backbone''' <BR>
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''DpnI digest of MMP2 insert and MMP2 Backbone'' <BR>
After the PCR of the previous days I digested both the MMP2 insert and Backbone for the Gibson Assembly.
After the PCR of the previous days I digested both the MMP2 insert and Backbone for the Gibson Assembly.
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''Gibson assembly for the MMP2+GFP construct'' <BR>
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'''Gibson assembly for the MMP2+GFP construct''' <BR>
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After the digest I did a Gibson assembly of the construct that contains the MMP2 gelatinase and superfolded GFP whose combined expression is induced by arabinose.
After the digest I did a Gibson assembly of the construct that contains the MMP2 gelatinase and superfolded GFP whose combined expression is induced by arabinose.
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''Transfromation of cells with the Cad-GFP Gibson Assembly Product (Sensing construct)'' <BR>
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'''Transfromation of cells with the Cad-GFP Gibson Assembly Product (Sensing construct)''' <BR>
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I transformed Cells with one cad-sensing Construct. They effectively grew, meaning that they did contain some sort of resistance. This indicated that the gibson had worked.
I transformed Cells with one cad-sensing Construct. They effectively grew, meaning that they did contain some sort of resistance. This indicated that the gibson had worked.
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Revision as of 16:40, 3 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Sensing

PCR of MMP9 insert and GelE insert
-I did the PCR of the MMP9 and the gelE insert which worked. I then purified it which gave me concentrations around 100ng/ul.

DpnI digest of MMP2 insert and MMP2 Backbone
After the PCR of the previous days I digested both the MMP2 insert and Backbone for the Gibson Assembly.

Gibson assembly for the MMP2+GFP construct
After the digest I did a Gibson assembly of the construct that contains the MMP2 gelatinase and superfolded GFP whose combined expression is induced by arabinose.

Transfromation of cells with the Cad-GFP Gibson Assembly Product (Sensing construct)
I transformed Cells with one cad-sensing Construct. They effectively grew, meaning that they did contain some sort of resistance. This indicated that the gibson had worked.

Wiki Links

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