Team:EPF Lausanne/Calendar/19 August 2013

From 2013.igem.org

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<font size = "4"> Sensing-Effector </font> <BR>
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Sensing <BR>
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''Gel electrophoresis of the backbones with and without GFP'' <BR>
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-After running the gel for 1.5h, there were two visible bands for the backbones but not at the right height. We were not sure if there was a problem with the ladder.
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'''Gel electrophoresis of the backbones with and without GFP''' <BR>
+
''Gradient PCR of inserts'' <BR>
-
After running the gel for 1.5h, there were two visible bands for the backbones but not at the right height. We were not sure if there was a problem with the ladder.
+
-As we were not sure about the annealing temperatures of the primers for the MMP inserts, we ran a gradient PCR for the MMP2 and MMP9 inserts with 8 different temperatures between 56°C and 65°C.
-
 
+
-
'''Gradient PCR of inserts''' <BR>
+
-
As we were not sure about the annealing temperatures of the primers for the MMP inserts, we ran a gradient PCR for the MMP2 and MMP9 inserts with 8 different temperatures between 56°C and 65°C.
+
This Gradient PCR worked very well, the inserts were amplified at almost all of the temperatures.
This Gradient PCR worked very well, the inserts were amplified at almost all of the temperatures.
 +
<BR> <BR>
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<font size = "4"> Nanoparticles</font> <BR>
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''Making Nanoparticles, 2nd try'' <BR>
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-Addition of bromophenol blue to the new nanoparticles to try and load them. The addition seems not to be working, since the solution precipitated.
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Latest revision as of 19:05, 3 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Sensing-Effector

Gel electrophoresis of the backbones with and without GFP
-After running the gel for 1.5h, there were two visible bands for the backbones but not at the right height. We were not sure if there was a problem with the ladder.

Gradient PCR of inserts
-As we were not sure about the annealing temperatures of the primers for the MMP inserts, we ran a gradient PCR for the MMP2 and MMP9 inserts with 8 different temperatures between 56°C and 65°C. This Gradient PCR worked very well, the inserts were amplified at almost all of the temperatures.

Nanoparticles

Making Nanoparticles, 2nd try
-Addition of bromophenol blue to the new nanoparticles to try and load them. The addition seems not to be working, since the solution precipitated.

Wiki Links

Retrieved from "http://2013.igem.org/Team:EPF_Lausanne/Calendar/19_August_2013"