Team:DTU-Denmark/Notebook/10 July 2013

From 2013.igem.org

(Difference between revisions)
(Run gel)
(Transformation in e.coli)
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* 3 samples from purification of 09.07.2013
* 3 samples from purification of 09.07.2013
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====Transformation in ''e.coli''====
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====Transformation of Biobricks====
According to the iGEM protocol ([http://parts.igem.org/Help:Protocols/Transformation transformation protocol]) with slight changes.
According to the iGEM protocol ([http://parts.igem.org/Help:Protocols/Transformation transformation protocol]) with slight changes.
* step 1: 100 μl cells  
* step 1: 100 μl cells  

Revision as of 16:15, 10 July 2013

Contents

208

Main purpose

Run gel with 9 PCR samples of Nir operon from Pseudomonas aeruginosa

Transformation in E.coli

Who was in the lab

Ariadni,Henrike,Julia,Natalia

Procedure

Run gel

  • 6 samples from PCR of 09.07.2013
  • ladder broadband
  • 3 samples from purification of 09.07.2013

Transformation of Biobricks

According to the iGEM protocol ([http://parts.igem.org/Help:Protocols/Transformation transformation protocol]) with slight changes.

  • step 1: 100 μl cells
  • step 2: 1.5 μl of the resuspended DNA
  • step 6: 90 sec (instead of 60 s)at 42 oC
  • step 9: Incubation without shaking

Results