Team:Tuebingen/Notebook/Protocols/plates

From 2013.igem.org

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     <td style="text-align: center">1 L</td>
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     <td style="text-align: center">1000 mL</td>
     <td><a href="/Team:Tuebingen/Notebook/Protocols/lb">LB medium</a></td>
     <td><a href="/Team:Tuebingen/Notebook/Protocols/lb">LB medium</a></td>
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    <td style="text-align: center">100 mL</td>
 
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    <td>EDTA (c = 0.5 M ; pH 8.0)</td>
 
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    <td style="text-align: center">10 mL</td>
 
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    <td>KCl (c = 250 mM)</td>
 
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<h3>Procedure</h3>
<h3>Procedure</h3>
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<p>Dissolve all reagents in 1000 mL Aqua bidest. Result: <b>50x concentration</b>.</p>
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<p>Add agar to LB medium and heat in microwave until all agar has completely dissolved. <u>Perform next steps at laminar flow cabinet!</u> Cast plates in petri dishes (approx. 20 mL per plate).</p>
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Revision as of 22:14, 3 October 2013

Return to iGEM Main Page.

LB Plates
Back to Protocols

 

Reagents

16.0 g Agar
1000 mL LB medium

 

Procedure

Add agar to LB medium and heat in microwave until all agar has completely dissolved. Perform next steps at laminar flow cabinet! Cast plates in petri dishes (approx. 20 mL per plate).