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Team:Tuebingen/Notebook/Protocols/ligation
From 2013.igem.org
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| - | <td style="text-align: center">5 µl (up to 5:1 molar ratio insert to vector) </td> | + | <td style="text-align: center">5 µl <br>(up to 5:1 molar ratio insert to vector) </td> |
<td>Insert DNA</td> | <td>Insert DNA</td> | ||
</tr> | </tr> | ||
Revision as of 00:18, 4 October 2013
Ligation
Back to Protocols
Reagents
| 20 - 50 ng | Linearized vector |
| 1 µL | 10x Ligase buffer |
| 1 µL | T4 Ligase |
| 5 µl (up to 5:1 molar ratio insert to vector) |
Insert DNA |
| to 10 µL | Aqua dest. |
Procedure
Dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. After autoclaving add 5 mL MgCL2 (c = 2 M).
