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Team:Tuebingen/Notebook/Protocols/lb
From 2013.igem.org
(Difference between revisions)
| Line 40: | Line 40: | ||
<td style="text-align: center">10.0 g</td> | <td style="text-align: center">10.0 g</td> | ||
<td>NaCl</td> | <td>NaCl</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center"></td> | ||
| + | <td>Antibiotics</td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
| Line 46: | Line 51: | ||
<h3>Procedure</h3> | <h3>Procedure</h3> | ||
| - | <p>Dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. Let cool down and add antibiotics if required.</p> | + | <p>Dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. Let cool down (LB-medium stays liqud) and add antibiotics if required. Store at 4 °C.</p> |
</div> | </div> | ||
Revision as of 02:53, 4 October 2013
LB Medium
Back to Protocols
Reagents
| 10.0 g | Trypton |
| 5.0 g | Yeast extract |
| 10.0 g | NaCl |
| Antibiotics |
Procedure
Dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. Let cool down (LB-medium stays liqud) and add antibiotics if required. Store at 4 °C.
