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Team:Tuebingen/Notebook/Protocols/tae
From 2013.igem.org
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| Line 51: | Line 51: | ||
<h3>Procedure</h3> | <h3>Procedure</h3> | ||
| - | <p>Dissolve all reagents in 1000 mL Aqua bidest. | + | <p>Dissolve all reagents in 1000 mL Aqua bidest. Store at room temperature. |
| + | <br>Procedure yields <b>50x concentration</b>.</p> | ||
</div> | </div> | ||
Revision as of 03:10, 4 October 2013
TAE Buffer
Back to Protocols
Reagents
| 242.0 g | Tris Base |
| 57.1 mL | Glacial acetic acid |
| 100 mL | EDTA (c = 0.5 M ; pH 8.0) |
| 10 mL | KCl (c = 250 mM) |
Procedure
Dissolve all reagents in 1000 mL Aqua bidest. Store at room temperature.
Procedure yields 50x concentration.
